1887

Abstract

Two novel, designated strains 29W222 and 2943, were isolated from the marine sediment from Aoshan Bay, Jimo, PR China. Growth was observed at pH 6.0–8.5 (optimum, pH 7.5) for strain 29W222, and pH 5.5–8.5 (pH 7.0) for strain 2943. Both strains displayed growth in 0.5–6 % NaCl with an optimum at 1 % for 29W222; 0.5 % for 2943. Both strains grew optimally at 33 °C. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that 29W222 and 2943 represented members of the genus and strain 29W222 was most closely related to KMM 6220 (97.9 % sequence similarity) and AK7 (95.0 %), and 2943 to AK7 (95.7 %) and KMM 6220 (94.8 %). The genomic DNA G+C contents of 29W222 and 2943 were 39.9 and 37.7 mol%, respectively. The results of chemotaxonomic analysis indicated that the sole respiratory quinone was menaquinone 7 (MK-7), and the major fatty acid was iso-C for both strains. Average nucleotide identity and average amino acid identity values between strain 29W222 and KMM 6220 were 78.9 and 83.6 %, respectively; the corresponding values between 2943 and AK7 were 69.8 and 63.6 %, respectively. Therefore, strains 29W222 and 2943 represent to two novel species of the genus , for which the names sp. nov. (29W222=KCTC 62848=MCCC 1K05194) and sp. nov. (2943=KCTC 62847= MCCC 1K05144) are proposed, respectively.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070002, 31770002)
    • Principle Award Recipient: Zong-JunDu
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2019FY100700)
    • Principle Award Recipient: Zong-JunDu
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005308
2022-03-22
2022-05-18
Loading full text...

Full text loading...

References

  1. Nedashkovskaya OI, Kim SB, Shin DS, Beleneva IA, Mikhailov VV. Fulvivirga kasyanovii gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from seawater in a mussel farm. Int J Syst Evol Microbiol 2007; 57:1046–1049 [View Article] [PubMed]
    [Google Scholar]
  2. Jung YT, Ha MJ, Park S, Lee JS, Yoon JH. Fulvivirga lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2016; 66:2604–2609 [View Article] [PubMed]
    [Google Scholar]
  3. Nupur Sharma S, Kumar Singh P, Suresh K, Anil Kumar P. Fulvivirga imtechensis sp. nov., a member of the phylum Bacteroidetes . Int J Syst Evol Microbiol 2012; 62:2213–2217 [View Article] [PubMed]
    [Google Scholar]
  4. Goldberg SR, Correa H, Haltli BA, Kerr RG. Fulvivirga aurantia sp. nov. and Xanthovirga aplysinae gen. nov., sp. nov., marine bacteria isolated from the sponge Aplysina fistularis, and emended description of the genus Fulvivirga . Int J Syst Evol Microbiol 2020; 70:2766–2781 [View Article]
    [Google Scholar]
  5. Wang ZJ, Xie ZH, Wang C, Du ZJ, Chen GJ. Motiliproteus sediminis gen. nov., sp. nov., isolated from coastal sediment. Antonie van Leeuwenhoek 2014; 106:615–621 [View Article]
    [Google Scholar]
  6. Mu D-S, Zhou L-Y, Liang Q-Y, Chen G-J, Du Z-J. Tichowtungia aerotolerans gen. nov., sp. nov., a novel representative of the phylum Kiritimatiellaeota and proposal of Tichowtungiaceae fam. nov., Tichowtungiales ord. nov. and Tichowtungiia class. nov. Int J Syst Evol Microbiol 2020; 70:5001–5011 [View Article] [PubMed]
    [Google Scholar]
  7. Magee CM, Rodeheaver G, Edgerton MT, Edlich RF. A more reliable Gram staining technic for diagnosis of surgical infections. Am J Surg 1975; 130:341–346 [View Article] [PubMed]
    [Google Scholar]
  8. Bernardet J-F, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article] [PubMed]
    [Google Scholar]
  9. Zhang J, Wang C, Han J-R, Chen G-J, Du Z-J. Alteromonas flava sp. nov. and Alteromonas facilis sp. nov., two novel copper tolerating bacteria isolated from a sea cucumber culture pond in China. Syst Appl Microbiol 2019; 42:217–222 [View Article]
    [Google Scholar]
  10. Biebl H, Pukall R, Lünsdorf H, Schulz S, Allgaier M et al. Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense . Int J Syst Evol Microbiol 2007; 57:1095–1107 [View Article]
    [Google Scholar]
  11. Du ZJ, Wang ZJ, Zhao JX, Chen GJ. Woeseia oceani gen. nov., sp. nov., a chemoheterotrophic member of the order Chromatiales, and proposal of Woeseiaceae fam. nov. Int J Syst Evol Microbiol 2016; 66:107–112 [View Article] [PubMed]
    [Google Scholar]
  12. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014; 64:1690–1696 [View Article]
    [Google Scholar]
  13. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans . Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article] [PubMed]
    [Google Scholar]
  14. Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article] [PubMed]
    [Google Scholar]
  15. Tamura K, Stecher G, Kumar S. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol 2021; 38:3022–3027 [View Article] [PubMed]
    [Google Scholar]
  16. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article] [PubMed]
    [Google Scholar]
  17. Li R, Zhu H, Ruan J, Qian W, Fang X et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010; 20:265–272 [View Article] [PubMed]
    [Google Scholar]
  18. Li R, Yu C, Li Y, Lam T-W, Yiu S-M et al. SOAP2: an improved ultrafast tool for short read alignment. Bioinformatics 2009; 25:1966–1967 [View Article] [PubMed]
    [Google Scholar]
  19. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 2008; 9:75 [View Article] [PubMed]
    [Google Scholar]
  20. Blin K, Shaw S, Kloosterman AM, Charlop-Powers Z, van Wezel GP et al. antiSMASH 6.0: improving cluster detection and comparison capabilities. Nucleic Acids Res 2021; 49:W29–W35 [View Article] [PubMed]
    [Google Scholar]
  21. Chaumeil PA, Mussig AJ, Hugenholtz P, Parks DH. GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database. Bioinformatics 20191925–1927 [View Article] [PubMed]
    [Google Scholar]
  22. Price MN, Dehal PS, Arkin AP. FastTree 2–approximately maximum-likelihood trees for large alignments. PLoS One 2010; 5:e9490 [View Article] [PubMed]
    [Google Scholar]
  23. Trifinopoulos J, Nguyen L-T, von Haeseler A, Minh BQ. W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Res 2016; 44:W232–5 [View Article] [PubMed]
    [Google Scholar]
  24. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article] [PubMed]
    [Google Scholar]
  25. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  26. Qin Q-L, Xie B-B, Zhang X-Y, Chen X-L, Zhou B-C et al. A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 2014; 196:2210–2215 [View Article] [PubMed]
    [Google Scholar]
  27. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
    [Google Scholar]
  28. Tindall BJ. Lipid-composition of Halobacterium lacusprofundii . FEMS Microbiol Lett 1990; 66:199–202
    [Google Scholar]
  29. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  30. Xin H, Itoh T, Zhou P, Suzuki K, Kamekura M et al. Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 2000; 50:1297–1303 [View Article] [PubMed]
    [Google Scholar]
  31. Dees SB, Carlone GM, Hollis D, Moss CW. Chemical and phenotypic characteristics of Flavobacterium thalpophilum compared with those of other Flavobacterium and Sphingobacterium species. Int J Syst Bacteriol 1985; 35:16–22 [View Article]
    [Google Scholar]
  32. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark, DE: MIDI; 1990
    [Google Scholar]
  33. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:1825 [View Article]
    [Google Scholar]
  34. Auch AF, von Jan M, Klenk H-P, Göker M. Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article] [PubMed]
    [Google Scholar]
  35. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005308
Loading
/content/journal/ijsem/10.1099/ijsem.0.005308
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error