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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 3

sp. nov., isolated from a flower of royal azalea () Free

Abstract

A Gram-negative, non-motile, strictly aerobic and rod- or filamentous-shaped strain, CJU-R4, was isolated from a flower of royal azalea () collected in the Republic of Korea. Strain CJU-R4 was catalase-positive and oxidase-negative, and grew at 15–33 °C (optimum, 28–20 °C), at pH 5.0–8.0 (optimum, pH 7.0–8.0), and in the presence of 0–1 % NaCl (w/v; optimum, 0 %). Strain CJU-R4 had the highest 16S rRNA gene sequence similarity to RHs22 (96.6 %), revealing less than 93 % sequence similarity to other type strains. Phylogenetic and phylogenomic analysis also revealed strain CJU-R4 formed a robust cluster with RHs22. The major fatty acids were summed feature 3 (comprising C 7 and/or C 6; 33.0 %), C 5 (22.1 %), iso-C (12.6 %) and C (10.7 %). The polar lipids were composed of phosphatidylethanolamine, three unidentified aminophospholipids, one unidentified phospholipid and four unidentified lipids. Menaquinone-7 was detected as the sole respiratory quinone. The genomic DNA G+C content was 55.2 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain CJU-R4 and DSM 28354 were 81.5 and 23.9 %, respectively. Based on the results of the phenotypic and genotypic analyses, strain CJU-R4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CJU-R4 (=KACC 21264=NBRC 114513).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , flower , polyphasic taxonomic , Rhododendron schlippenbachii and Spirosoma rhododendri sp. nov.
Funding
This study was supported by the:
  • National Institute of Agricultural Sciences (Award PJ013549)
    • Principal Award Recipient: Soon-WoKwon
© 2022 The Authors
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2022-03-29
2026-02-10

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Spirosoma rhododendri sp. nov., isolated from a flower of royal azalea (Rhododendron schlippenbachii)
Int J Syst Evol Microbiol 72, 005306 (2022); https://doi.org/10.1099/ijsem.0.005306
/content/journal/ijsem/10.1099/ijsem.0.005306
/content/journal/ijsem/10.1099/ijsem.0.005306
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