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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 4

sp. nov., a novel lignin-degrading actinobacterium isolated from rhizosphere soil of pumpkin No Access

Abstract

A novel lignin-degrading actinobacterium, designated NEAU-G5, was isolated from pumpkin rhizosphere soil collected from field in Mudanjiang, Heilongjiang Province, northeast China, and characterized using polyphasic approach. The prior 16S rRNA gene sequence similarities and phylogenic analysis showed that strain NEAU-G5 exhibited close phylogenetic relatedness to NBRC 108239 (98.82 %), NBRC 15556 (98.75 %), NBRC 101359 (98.68 %) and RB20 (98.61 %). Morphological and chemotaxonomic characteristics indicated that strain NEAU-G5 could be assigned to the genus . The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The predominant menaquinone was MK-8(H, ω-cycl). The major fatty acids (>10 %) were identified as C, C 9, 10-methyl C and C. Mycolic acids were present. The genomic DNA G+C content of strain NEAU-G5 was 68 mol%. Moreover, based on digital DNA–DNA hybridization and average nucleotide identity values, strain NEAU-G5 could be differentiated from its reference strains. In addition, an azure B plate decolorization test and genomic analysis indicated that strain NEAU-G5 had the ability to degrade lignin. On the basis of polyphasic characteristics, strain NEAU-G5 represents a novel species of the genus , with the name sp. nov. The type strain is NEAU-G5 (=CCTCC AA 2021018=DSM 110547).

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  • Accepted:
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Keyword(s): 16S rRNA gene , genome , lignin-degrading actinobacterium , Nocardia albiluteola sp. nov. , polyphasic approach and rhizosphere soil of pumpkin
Funding
This study was supported by the:
  • the Key Program of the National Natural Science Foundation of China (Award 32030090)
    • Principle Award Recipient: WenshengXiang
© 2022 The Authors
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2022-04-12
2024-04-19
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Nocardia albiluteola sp. nov., a novel lignin-degrading actinobacterium isolated from rhizosphere soil of pumpkin
Int J Syst Evol Microbiol 72, 005305 (2022); https://doi.org/10.1099/ijsem.0.005305
/content/journal/ijsem/10.1099/ijsem.0.005305
/content/journal/ijsem/10.1099/ijsem.0.005305
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