1887

Abstract

Two Gram-stain-negative, strictly aerobic bacteria, strains L1-7-SE and R6, isolated from marine red algae, were characterized. They shared 99.9 % 16S rRNA gene sequence similarity and a 100 % digital DNA–DNA hybridization (DDH) value, representing members of a single species. Cells of strains L1-7-SE and R6 were catalase- and oxidase-positive motile rods with a single polar flagellum. Strains L1-7-SE and R6 optimally grew at 30–35 °C, pH 7.0–8.0 and with 1.0–2.0 % (w/v) NaCl. Ubiquinone-10 was the sole isoprenoid quinone and C cyclo 8 and summed feature 8 (comprising C 7 and/or C 6) were detected as the major cellular fatty acids. The DNA G+C contents of strains L1-7-SE and R6 were both 61.62 mol%. The polar lipids of strain L1-7-SE consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and two unidentified polar lipids. Phylogenetic analyses based on 16S rRNA gene and 120 protein marker sequences revealed that strains L1-7-SE and R6 formed a phyletic lineage within the genus and they were most closely related to NL21 and KY 101 with both 98.8 % 16S rRNA gene sequence similarities. Digital DDH values between strain L1-7-SE and the type strains of and were 60.3 and 29.5 %, respectively. The phenotypic, chemotaxonomic and molecular features support that strains L1-7-SE and R6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is L1-7-SE (=KACC 19076=KCTC 92231=JCM 31802).

Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR202029201)
    • Principle Award Recipient: CheOk Jeon
  • Ocean Science & Technology (Award KIOST20210469)
    • Principle Award Recipient: CheOk Jeon
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005302
2022-03-28
2022-05-18
Loading full text...

Full text loading...

References

  1. Labbé N, Parent S, Villemur R. Nitratireductor aquibiodomus gen. nov., sp. nov., a novel alpha-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). Int J Syst Evol Microbiol 2004; 54:269–273 [View Article]
    [Google Scholar]
  2. Kim K-H, Roh SW, Chang H-W, Nam Y-D, Yoon J-H et al. Nitratireductor basaltis sp. nov., isolated from black beach sand. Int J Syst Evol Microbiol 2009; 59:135–138 [View Article]
    [Google Scholar]
  3. Kang HS, Yang HL, Lee SD. Nitratireductor kimnyeongensis sp. nov., isolated from seaweed. Int J Syst Evol Microbiol 2009; 59:1036–1039 [View Article]
    [Google Scholar]
  4. Jang GI, Hwang CY, Cho BC. Nitratireductor aquimarinus sp. nov., isolated from a culture of the diatom skeletonema costatum, and emended description of the genus Nitratireductor. Int J Syst Evol Microbiol 2011; 61:2676–2681
    [Google Scholar]
  5. Jiang Z, Duan Y, Yang X, Yao B, Zeng T et al. Nitratireductor alexandrii sp. nov., from phycosphere microbiota of toxic marine dinoflagellate Alexandrium tamarense. Int J Syst Evol Microbiol 2020; 70:4390–4397 [View Article]
    [Google Scholar]
  6. Lai Q, Yu Z, Yuan J, Sun F, Shao Z. Nitratireductor indicus sp. nov., isolated from deep-sea water. Int J Syst Evol Microbiol 2011; 61:295–298 [View Article] [PubMed]
    [Google Scholar]
  7. Ou D, Huang H, Bai R, Li Q, Wang Y et al. Nitratireductor aestuarii sp. nov., a marine alphaproteobacterium isolated from an estuary. Int J Syst Evol Microbiol 2017; 67:1637–1642 [View Article] [PubMed]
    [Google Scholar]
  8. Kim J, Kim KH, Chun BH, Khan SA, Jeon CO. Flagellimonas algicola sp. nov., isolated from a marine Red Alga, Asparagopsis taxiformis. Curr Microbiol 2020; 77:294–299 [View Article] [PubMed]
    [Google Scholar]
  9. Khan SA, Jeong SE, Baek JH, Jeon CO. Maribacter algicola sp. nov., isolated from a marine red alga, Porphyridium marinum, and transfer of Maripseudobacter aurantiacus Chen et al. 2017 to the genus Maribacter as Maribacter aurantiacus comb. nov. Int J Syst Evol Microbiol 2020; 70:797–804 [View Article]
    [Google Scholar]
  10. Jeong SE, Kim KH, Lhee D, Yoon HS, Quan ZX et al. Oceaniradius stylonematis gen. nov., sp. nov., isolated from a red alga, Stylonema cornu-cervi. Int J Syst Evol Microbiol 2019; 69:1967–1973 [View Article]
    [Google Scholar]
  11. Murphy CD, Moore RM, White RL. Peroxidases from marine microalgae. J Appl Phycol 2000; 12:507–513 [View Article]
    [Google Scholar]
  12. Kim JM, Le NT, Chung BS, Park JH, Bae J-W et al. Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 2008; 74:7313–7320 [View Article] [PubMed]
    [Google Scholar]
  13. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article] [PubMed]
    [Google Scholar]
  14. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007; 3:e56 [View Article] [PubMed]
    [Google Scholar]
  15. Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 2021; 38:3022–3027 [View Article]
    [Google Scholar]
  16. Kim KR, Kim KH, Khan SA, Kim HM, Han DM et al. Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. isolated from soil. J Microbiol 2021; 59:709–718 [View Article]
    [Google Scholar]
  17. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1989
    [Google Scholar]
  18. Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: resolvingResolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 2017; 13:e1005595 [View Article] [PubMed]
    [Google Scholar]
  19. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article] [PubMed]
    [Google Scholar]
  20. Chaumeil PA, Mussig AJ, Hugenholtz P, Parks DH. GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database. Bioinformatics 2019; 36:1925–1927 [View Article] [PubMed]
    [Google Scholar]
  21. Lee I, Ouk Kim Y, Park S-C, Chun J. OrthoANI: An improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article] [PubMed]
    [Google Scholar]
  22. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  23. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kämpfer P et al. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 2002; 52:1043–1047 [View Article] [PubMed]
    [Google Scholar]
  24. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article] [PubMed]
    [Google Scholar]
  25. Amin SA, Hmelo LR, van Tol HM, Durham BP, Carlson LT et al. Interaction and signalling between a cosmopolitan phytoplankton and associated bacteria. Nature 2015; 522:98–101 [View Article] [PubMed]
    [Google Scholar]
  26. Croft MT, Lawrence AD, Raux-Deery E, Warren MJ, Smith AG. Algae acquire vitamin B12 through a symbiotic relationship with bacteria. Nature 2005; 438:90–93 [View Article] [PubMed]
    [Google Scholar]
  27. van Tol HM, Amin SA, Armbrust EV. Ubiquitous marine bacterium inhibits diatom cell division. ISME J 2017; 11:31–42 [View Article] [PubMed]
    [Google Scholar]
  28. Oren A. Halophilic microbial communities and their environments. Curr Opin Biotechnol 2015; 33:119–124 [View Article] [PubMed]
    [Google Scholar]
  29. Gevrekci . The roles of polyamines in microorganisms. World J Microbiol Biotechnol 2017; 33:204 [View Article] [PubMed]
    [Google Scholar]
  30. Roberts MF. Organic compatible solutes of halotolerant and halophilic microorganisms. Saline Syst 2005; 1:1–30 [View Article] [PubMed]
    [Google Scholar]
  31. Gomori G. Preparation of buffers for use in enzyme studies. Methods Enzymol 1955; 1:138–146
    [Google Scholar]
  32. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67
    [Google Scholar]
  33. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. eds Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994607–654
    [Google Scholar]
  34. Komagata K, Suzuki KI. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–208
    [Google Scholar]
  35. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty acids, MIDI Technical note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  36. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
    [Google Scholar]
  37. Yu Z, Zhuang L, Pan J, Wang Y, Zhou S. Nitratireductor lacus sp. nov., isolated from Yuncheng Salt Lake, China. Int J Syst Evol Microbiol 2016; 66:4963–4967 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005302
Loading
/content/journal/ijsem/10.1099/ijsem.0.005302
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error