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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 3

sp. nov., isolated from deep-sea sponge No Access

Abstract

A novel bacterial strain (MSC19) was isolated from a deep-sea sponge collected in the Mariana Trench at a depth of 2681 m. The cells of the new isolate were Gram-stain-positive, non-motile, oxidase- and catalase-positive, rod-shaped and yellow-coloured. They could grow at 4–32 °C (optimum, 28 °C), pH 5.5–12 (optimum, pH 7.0) and with 0–12 % (w/v) NaCl (optimum, 4 %). The strain’s 16S rRNA gene sequence showed 98.41 % similarity to that of CM-01. Phylogenetic analysis further suggested that strain MSC19 represents a new species within the genus . The total genome of MSC19 was approximately 3 196 754 bp in size with a G+C content of 66.43 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values among MSC19 and other type strains were 70.35–75.37 % (ANIb), 83.79–84.73 % (ANIm) and 20.3–21.7 % (dDDH). The major fatty acids of MSC19 were composed of anteiso-C, iso-C and anteiso-C, and its predominant menaquinones were MK-10 and MK-9. The polar lipids of MSC19 mainly consisted of diphosphatidylglycerol, phosphatidylglycerol and glycolipid. The diagnostic cell-wall diamino acid was lysine. Combined molecular, physiological, biochemical and chemotaxonomic analyses suggest that strain MSC19 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MSC19 (=MCCC 1K06265=KCTC 49701).

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Keyword(s): deep-sea sponge , Mycetocola spongiae and polyphasic taxonomy
Funding
This study was supported by the:
  • National Key Research and Development Program of China (Award 2018YFA0901901)
    • Principle Award Recipient: ZhiyongLi
© 2022 The Authors
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2022-03-22
2025-01-13
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Mycetocola spongiae sp. nov., isolated from deep-sea sponge Cacospongia mycofijiensis
Int J Syst Evol Microbiol 72, 005291 (2022); https://doi.org/10.1099/ijsem.0.005291
/content/journal/ijsem/10.1099/ijsem.0.005291
/content/journal/ijsem/10.1099/ijsem.0.005291
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