sp. nov., isolated from bulk tank raw milk at a German dairy farm Open Access

Abstract

Here we present the description of a novel species, designated sp. nov., which was isolated from raw milk samples obtained from Germany. Results of initial 16S rRNA gene sequence analysis assigned the strain into the genus and showed , and to be its closest relatives. Further studies including sequence analysis of the gene, multi-gene phylogenetic tree reconstruction, whole-genome sequence comparisons, cellular fatty acid analysis and chemotaxonomic characterization showed a clear separation from the known species. Isolate MBT-4 was closely related to , ' and with average nucleotide identities based on values of 88.8, 88.8 and 88.6%, respectively. Therefore, the strain can be classified into the subgroup of the group. The G+C content of strain MBT-4 was 58.9 mol%. The strain was catalase- and oxidase-positive, while the β-galactosidase reaction was negative. Growth occurred between 4 and 30 °C and at pH values from pH 6.0 to 8.0. In conclusion, strain MBT-4 belongs to a novel species, for which the name sp. nov. is proposed. The type strain is MBT-4 (=DSM 112348=LMG 32241) and strain MBT-17 is also a representative of this species.

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2022-04-05
2024-03-28
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