1887

Abstract

Here we present the description of a novel species, designated sp. nov., which was isolated from raw milk samples obtained from Germany. Results of initial 16S rRNA gene sequence analysis assigned the strain into the genus and showed , and to be its closest relatives. Further studies including sequence analysis of the gene, multi-gene phylogenetic tree reconstruction, whole-genome sequence comparisons, cellular fatty acid analysis and chemotaxonomic characterization showed a clear separation from the known species. Isolate MBT-4 was closely related to , ' and with average nucleotide identities based on values of 88.8, 88.8 and 88.6%, respectively. Therefore, the strain can be classified into the subgroup of the group. The G+C content of strain MBT-4 was 58.9 mol%. The strain was catalase- and oxidase-positive, while the β-galactosidase reaction was negative. Growth occurred between 4 and 30 °C and at pH values from pH 6.0 to 8.0. In conclusion, strain MBT-4 belongs to a novel species, for which the name sp. nov. is proposed. The type strain is MBT-4 (=DSM 112348=LMG 32241) and strain MBT-17 is also a representative of this species.

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2022-04-05
2022-05-18
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References

  1. Parte AC, Sardà Carbasse J, Meier-Kolthoff JP, Reimer LC, Göker M. List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. Int J Syst Evol Microbiol 2020; 70:5607–5612 [View Article] [PubMed]
    [Google Scholar]
  2. Affolter VK, Moore PF et al. Feline progressive histiocytosis. Vet Pathol 2006; 43:646–655 [View Article] [PubMed]
    [Google Scholar]
  3. Hantsis-Zacharov E, Halpern M. Culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits. Appl Environ Microbiol 2007; 73:7162–7168 [View Article] [PubMed]
    [Google Scholar]
  4. Boor KJ, Wiedmann M, Murphy S, Alcaine S. A 100-Year Review: Microbiology and safety of milk handling. J Dairy Sci 2017; 100:9933–9951 [View Article] [PubMed]
    [Google Scholar]
  5. Murphy SC, Boor KJ. Trouble-shooting sources and causes of high bacteria counts in raw milk dairy. Food Environ Sanit 2000; 20:606–611
    [Google Scholar]
  6. von Neubeck M, Baur C, Krewinkel M, Stoeckel M, Kranz B et al. Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential. Int J Food Microbiol 2015; 211:57–65 [View Article] [PubMed]
    [Google Scholar]
  7. von Neubeck M, Huptas C, Glück C, Krewinkel M, Stoeckel M et al. Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow’s milk. Int J Syst Evol Microbiol 2016; 66:1163–1173 [View Article]
    [Google Scholar]
  8. von Neubeck M, Huptas C, Glück C, Krewinkel M, Stoeckel M et al. Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk. Int J Syst Evol Microbiol 2017; 67:1656–1664 [View Article]
    [Google Scholar]
  9. Hofmann K, Huptas C, Doll EV, Scherer S, Wenning M. Pseudomonas haemolytica sp. nov., isolated from raw milk and skimmed milk concentrate. Int J Syst Evol Microbiol 2020; 70:2339–2347 [View Article] [PubMed]
    [Google Scholar]
  10. Hofmann K, Woller A, Huptas C, Wenning M, Scherer S et al. Pseudomonas cremoris sp. nov., a novel proteolytic species isolated from cream. Int J Syst Evol Microbiol 2021; 71:004597 [View Article] [PubMed]
    [Google Scholar]
  11. Hofmann K, Huptas C, Doll EV, Scherer S, Wenning M. Pseudomonas saxonica sp. nov., isolated from raw milk and skimmed milk concentrate. Int J Syst Evol Microbiol 2020; 70:935–943 [View Article] [PubMed]
    [Google Scholar]
  12. Ribeiro Júnior JC, de Oliveira AM, Silva F de G, Tamanini R, de Oliveira ALM et al. The main spoilage-related psychrotrophic bacteria in refrigerated raw milk. J Dairy Sci 2018; 101:75–83 [View Article] [PubMed]
    [Google Scholar]
  13. Gieschler S, Fiedler G, Böhnlein C, Grimmler C, Franz C et al. Pseudomonas kielensis sp. nov. and pseudomonas baltica sp. nov., isolated from raw milk in germany. Int J Syst Evol Microbiol 2019; 71:004717 [View Article]
    [Google Scholar]
  14. Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 2017; 13:e1005595 [View Article] [PubMed]
    [Google Scholar]
  15. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP et al. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 2016; 44:6614–6624 [View Article] [PubMed]
    [Google Scholar]
  16. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article] [PubMed]
    [Google Scholar]
  17. Lalucat J, Mulet M, Gomila M, García-Valdés E. Genomics in bacterial taxonomy: impact on the genus Pseudomonas. Genes 2020; 11:E139 [View Article] [PubMed]
    [Google Scholar]
  18. Moore ERB, Mau M, Arnscheidt A, Böttger EC, Hutson RA et al. The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu stricto and estimation of the natural intrageneric relationships. Syst Appl Microbiol 1996; 19:478–492 [View Article]
    [Google Scholar]
  19. Gomila M, Peña A, Mulet M, Lalucat J, García-Valdés E. Phylogenomics and systematics in Pseudomonas. Front Microbiol 2015; 6:214 [View Article] [PubMed]
    [Google Scholar]
  20. Meier-Kolthoff JP, Göker M. TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy. Nat Commun 2019; 10:2182 [View Article] [PubMed]
    [Google Scholar]
  21. Schlusselhuber M, Girard L, Cousin FJ, Lood C, De Mot R et al. Pseudomonas crudilactis sp. nov., isolated from raw milk in France. Antonie van Leeuwenhoek 2021; 114:719–730 [View Article]
    [Google Scholar]
  22. Duman M, Mulet M, Altun S, Burcin Saticioglu I, Gomila M et al. Pseudomonas anatoliensis sp. nov and Pseudomonas iridis sp. nov. isolated from fish. Syst Appl Microbiol 2021; 44:126198 [View Article] [PubMed]
    [Google Scholar]
  23. Morimoto Y, Lu YJ, Zuo H, Aibibula Z, Tohya M et al. Pseudomonas allokribbensis sp. nov. and Pseudomonas gozinkensis sp. nov., two new species isolated from a volcanic Island, Izu Oshima, Japan. Curr Microbiol 2021; 78:1670–1677 [View Article] [PubMed]
    [Google Scholar]
  24. Richter M, Rosselló-Móra R, Oliver Glöckner F, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 2016; 32:929–931 [View Article] [PubMed]
    [Google Scholar]
  25. Wattam AR, Davis JJ, Assaf R, Boisvert S, Brettin T et al. Improvements to PATRIC, the all-bacterial bioinformatics database and analysis resource center. Nucleic Acids Res 2017; 45:D535–D542 [View Article] [PubMed]
    [Google Scholar]
  26. Morimoto Y, Uwabe K, Tohya M, Hiramatsu K, Kirikae T et al. Pseudomonas atagosis sp. nov., and Pseudomonas akappagea sp. nov., new soil bacteria isolated from samples on the Volcanic Island Izu Oshima, Tokyo. Curr Microbiol 2020; 77:1909–1915 [View Article]
    [Google Scholar]
  27. Duman M, Mulet M, Altun S, Saticioglu IB, Gomila M et al. Corrigendum: Pseudomonas piscium sp. nov., Pseudomonas pisciculturae sp. nov., Pseudomonas mucoides sp. nov. and Pseudomonas neuropathica sp. nov. isolated from rainbow trout. Int J Syst Evol Microbiol 2021; 71: [View Article]
    [Google Scholar]
  28. Sørensen MCH, Gencay YE, Birk T, Baldvinsson SB, Jäckel C et al. Primary isolation strain determines both phage type and receptors recognised by Campylobacter jejuni bacteriophages. PLoS One 2015; 10:e0116287 [View Article] [PubMed]
    [Google Scholar]
  29. Danylec N, Göbl A, Stoll DA, Hetzer B, Kulling SE et al. Rubneribacter badeniensis gen. nov., sp. nov. and Enteroscipio rubneri gen. nov., sp. nov., new members of the Eggerthellaceae isolated from human faeces. Int J Syst Evol Microbiol 2018; 68:1533–1540 [View Article]
    [Google Scholar]
  30. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16:584–586 [View Article] [PubMed]
    [Google Scholar]
  31. Kuykendall LD, Roy MA, O’Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology gAcids, Antibiotic Resistance, and Deoxyribonucleic Acid Homology Groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 1988; 38:358–361 [View Article]
    [Google Scholar]
  32. Ramírez-Bahena M-H, Cuesta MJ, Flores-Félix JD, Mulas R, Rivas R et al. Pseudomonas helmanticensis sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 2014; 64:2338–2345 [View Article]
    [Google Scholar]
  33. Pascual J, García-López M, Bills GF, Genilloud O. Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain. Int J Syst Evol Microbiol 2015; 65:625–632 [View Article] [PubMed]
    [Google Scholar]
  34. Tvrzová L, Schumann P, Spröer C, Sedláček I, Páčová Z et al. Pseudomonas moraviensis sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of Pseudomonas asplenii. Int J Syst Evol Microbiol 2006; 56:2657–2663 [View Article]
    [Google Scholar]
  35. López JR, Diéguez AL, Doce A, De la Roca E, De la Herran R et al. Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneata (Moreau). Int J Syst Evol Microbiol 2012; 62:874–882 [View Article]
    [Google Scholar]
  36. Kwon SW, Kim JS, Park IC, Yoon SH, Park DH et al. Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 2003; 53:21–27 [View Article]
    [Google Scholar]
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