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Volume 72, Issue 3

sp. nov. and sp. nov., two acidophilic bacteria isolated from acid mine drainage Open Access

Abstract

Two acidophilic strains, designated as ALEF1 and S30H14, were isolated from acid mine drainage sediment. Cells of both strains were Gram-stain-positive, aerobic, endospore-forming rods. Strains ALEF1 and S30H14 were acidophilic and mesophilic, the former grew at 20–40 °C (optimum, 30 °C) and pH 2.5–4.5 (optimum, pH 3.5), while the latter grew at 20–45 °C (optimum, 30 °C) and pH 2.0–5.5 (optimum, pH 4.5). The 16S rRNA gene-based sequence analysis revealed that strains ALEF1 and S30H14 belonged to the genus , and were phylogenetically close to TC-34 with 97.1 and 97.4% similarity, respectively. The similarity between the two novel strains was 98.6 %. The average nucleotide identity value between the genome sequences of ALEF1 and S30H14 was 79.5 %, and that between each of the two isolates and TC-34 were 72.0 and 74.3 %. In addition, the digital DNA–DNA hybridization value between ALEF1 and S30H14 was 24.9 %, between strain ALEF1 and TC-34 was 21.7 %, and between S30H14 and TC-34 was 26.3 %, far below the interspecies threshold. Both strains could utilize diverse carbon sources for heterotrophic growth; strain ALEF1 could utilize ferrous iron as the energy source for autotrophic growth. Menaquinone 7 was the only quinone detected in either strain. Both strains contained anteiso-C and anteiso-C, while -alicyclic fatty acids were not detected. Based on their phylogenetic positions, as well as phenotypic and genomic data, it is considered that strains ALEF1 and S30H14 represent two novel species within the genus , for which the names sp. nov. (type strain ALEF1=CGMCC 1.17055=KCTC 43124) and sp. nov. (S30H14=CGMCC 1.17050=KCTC 43125) are proposed.

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Keyword(s): acid mine drainage , acidophile , Alicyclobacillus curvatus and Alicyclobacillus mengziensis
Funding
This study was supported by the:
  • CAS-NSTDA Joint Research Program (Award NO. 153211KYSB20200039)
    • Principle Award Recipient: Cheng-YingJiang
  • National Nature Science Foundation of China (Award 31670124)
    • Principle Award Recipient: Cheng-YingJiang
  • Innovation Academy for Green Manufacture, Chinese Academy of Sciences (Award IAGM2020C24)
    • Principle Award Recipient: Cheng-YingJiang
  • CAS Engineering Laboratory for Advanced Microbial Technology of Agriculture, Chinese Academy of Sciences (Award KFJ-PTXM-016)
    • Principle Award Recipient: Cheng-YingJiang
  • Major Research Plan of National Natural Science Foundation of China (Award 91851206)
    • Principle Award Recipient: Cheng-YingJiang
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-03-21
2024-04-19
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Alicyclobacillus curvatus sp. nov. and Alicyclobacillus mengziensis sp. nov., two acidophilic bacteria isolated from acid mine drainage
Int J Syst Evol Microbiol 72, 005285 (2022); https://doi.org/10.1099/ijsem.0.005285
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