1887

Abstract

The genus plays an important role in polysaccharide degradation and fermentation in the rumen. To further understand the function of the phylogenetically diverse genus , it is necessary to explore the individual characteristics at the species level. In this study, Gram-negative anaerobic bacterial strains isolated from the rumen of Holstein cows were identified. Strain R5019 was classified within the genus based on 16S rRNA gene sequence-based phylogenetic analysis. The values of 16S rRNA gene sequence similarity, average nucleotide identity and digital DNA–DNA hybridization between strain R5019 and its phylogenetically nearest species PPPA20 were 89.8, 82.6, and 29.3 %, respectively. The genome size of R5019 was estimated to be ca. 4.19 Mb with a genomic G+C content of 49.5 mol%. The major cellular fatty acids and menaquinones were C anteiso and C anteiso and MK-11 and MK-12, respectively. Succinate, lactate, malate, acetate and formate were produced as the fermentation end products using glucose. Based on phylogenetic, physiological, biochemical and genomic differences between 11 strains and other phylogenetically related species, a novel species, sp. nov., is proposed within the genus . The type strain is R5019 (=JCM 34664=DSM 112675).

Keyword(s): cow , Prevotella and rumen
Funding
This study was supported by the:
  • Ministry of Agriculture, Forestry and Fisheries (Award NA)
    • Principle Award Recipient: TakumiShinkai
  • the Cabinet Office, Government of Japan (Award No. JPJ009237)
    • Principle Award Recipient: TakumiShinkai
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2022-03-07
2022-07-06
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