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Abstract

A novel bacterium, designated SCR006, was isolated from tidal flat sediment from Suncheon Bay, Republic of Korea. Cells of strain SCR006 were strictly anaerobic, motile cocci, Gram-reaction-negative, and catalase- and oxidase-negative. Growth was observed at 4–41 °C (optimum, 34–37 °C), at pH 6.5–10.0 (optimum, pH 7.0–7.5) and in presence of 0–8 % NaCl (optimum, 0–2 %). Fermentation products of peptone–yeast–glucose medium were acetate and ethanol. Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SCR006 had high sequence similarity to D3RC-2 (97.9 %), followed by DSM 6139 (95.9 %) and 232.1 (95.0 %). The average nucleotide identity value between strain SCR006 and DSM 24773 was 72.7 %, which strongly supported that strain SCR006 reresents a novel species within the genus . The major cellular fatty acids are iso-C (27.2 %) and anteiso-C (16.9 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, an unidentified aminolipid and five unidentified lipids. The genomic size was 3.2 Mb with genomic DNA G+C content of 45.6 mol%. The results of 16S rRNA-based and genome-based phylogenetic tree analyses indicated that SCR006 should be assigned to the genus . Strain SCR006 could be distinguished from D3RC-2 by its growth conditions, cell morphology and genomic characteristics. Based on the phenotypic, phylogenetic, genomic and chemotaxonomic features, strain SCR006 represents a novel species, for which the name sp. nov. is proposed, with the type strain SCR006 (=KCTC 25245= JCM 34531)

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2022-04-08
2022-07-06
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