1887

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Volume 72, Issue 3

sp. nov. and sp. nov., two new endophytic bacterial species isolated from plant roots No Access

Abstract

Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103 and DLE-14 were isolated from plant roots. The 16S rRNA gene sequence analysis indicated that strain MWE-103 was closely related to SY01 with a sequence similarity of 97.82 %, and strain DLE-14 to IZS3-5 with 99.09 % similarity. The orthologous average nucleotide identity and digital DNA–DNA hybridization values using whole genome data indicated that strains MWE-103 and DLE-14 could be readily distinguished from the mostly related species. Both strains grew at mesophilic temperature ranges, and grew best at pH 6 and in the absence of NaCl. The major fatty acid in both strains was anteiso-C, but their relative proportions differed. The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was -diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species. Amylase and cellulase activities were positive for both strains. Strain DLE-14 exhibited the potential for lignin degradation. The DNA G+C contents of strain MWE-103 and DLE-14 were 60.9 and 50.8 mol% respectively. The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization. Based on phylogenetic and phenotypic evidence, strains MWE-103 and DLE-14 should each be recognized as a novel species of , for which the names sp. nov. (type strain: MWE-103=KCTC 43287=JCM 34503) and sp. nov. (type strain: DLE-14=KCTC 43288=JCM 34504) are proposed.

  • Received:
  • Accepted:
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Keyword(s): Artemisia princeps , Hemerocallis , lignin degradation , Paenibacillus artemisiicola , Paenibacillus lignilyticus and root endophyte
Funding
This study was supported by the:
  • National Institute of Biological Resources
    • Principle Award Recipient: YouJu Ham
© 2022 The Authors
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2022-03-02
2024-04-25
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Paenibacillus artemisiicola sp. nov. and Paenibacillus lignilyticus sp. nov., two new endophytic bacterial species isolated from plant roots
Int J Syst Evol Microbiol 72, 005270 (2022); https://doi.org/10.1099/ijsem.0.005270
/content/journal/ijsem/10.1099/ijsem.0.005270
/content/journal/ijsem/10.1099/ijsem.0.005270
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