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Volume 72, Issue 2

sp. nov., a moderately thermophilic bacterium isolated from a hot spring microbial mat No Access

Abstract

Three closely related, facultative anaerobic, Gram-stain-negative, twitching motile, short rod-shaped, non-endospore-forming, moderately thermophilic bacteria, designated strains SYSU G05001, SYSU G05003 and SYSU G05004, were isolated from a hot spring microbial mat, collected from Rehai National Park, Tengchong, Yunnan Province, south-western China. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that these three strains were closely related to SE-1 (97.97, 98.18, 97.90 % sequence similarity). Whole genome sequencing and polyphasic taxonomic approach were used to determine the genomic profile and taxonomic status of the novel strain SYSU G05001. Cell growth occurred at 37–80 °C (optimum, 55 °C), pH 6.0–8.0 (optimum, pH 7.0) and with 0–3.0 % (w/v) NaCl (optimum, 1%). Thiosulfate enhanced cell growth. MK-8 was the predominant menaquinone. The major cellular fatty acids included iso-C, iso-C and anteiso-C. The major polar lipids were consisted of aminophospholipid, glycolipid and phospholipids. The whole genome of strain SYSU G05001 consisted of 2.55 Mbp and the DNA G+C content was 64.94 mol%. The average nucleotide identity (≤94.95 %) and digital DNA–DNA hybridization (≤62.3 %) values between strain SYSU G05001 and other members of the genus were all lower than the threshold values recommended for distinguishing novel prokaryotic species. On the basis of the presented polyphasic evidence and genotypic data, it is proposed that strain SYSU G05001 (=KCTC 82627=MCCC 1K06118) represents a novel species of the genus , for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): hot spring , polyphasic taxonomy , Themurs brevis sp. nov. and Yunnan
Funding
This study was supported by the:
  • China Postdoctoral Science Foundation (Award 2020M672948)
    • Principle Award Recipient: ShaTan
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: Jian-YuJiao
  • National Natural Science Foundation of China (Award 31970122)
    • Principle Award Recipient: En-MinZhou
  • National Natural Science Foundation of China (Award 32100042)
    • Principle Award Recipient: ShaTan
  • National Natural Science Foundation of China (Award 32170101)
    • Principle Award Recipient: Wen-DongXian
  • National Natural Science Foundation of China (Award 91951205)
    • Principle Award Recipient: Wen-JunLi
  • Guangdong Basic and Applied Basic Research Foundation (Award 2019A1515110227)
    • Principle Award Recipient: LanLiu
© 2022 The Authors
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Thermus brevis sp. nov., a moderately thermophilic bacterium isolated from a hot spring microbial mat
Int J Syst Evol Microbiol 72, 005265 (2022); https://doi.org/10.1099/ijsem.0.005265
/content/journal/ijsem/10.1099/ijsem.0.005265
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