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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 3

sp. nov., a haloalkaliphilic bacterium isolated from a soda lake in Inner Mongolia Autonomous Region, China Open Access

Abstract

A haloalkaliphilic strain (IM 1326) was isolated from brine sampled at a soda lake in the Inner Mongolia Autonomous Region, China. Cells of the strain were rod-shaped and motile. Strain IM 1326 was able to grow at 4–42 °C (optimum, 37 °C) with 0–13.0 % (w/v) NaCl concentrations (optimum at 4.0–6.0 %) and at pH 7.5–11.0 (optimum at 9.0–10.0). The 16S rRNA gene phylogenetic analysis revealed that the isolate belongs to the genus and is closely related to the type strains of (95.8 % sequence similarity), (95.7 %), (95.4 %) and (95.3 %). The whole genome of strain IM 1326 was sequenced, and the genomic DNA G+C content was 49.7 mol%. Average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between the isolate and the related species were 68.1–84.9 %, 76–78 % and 18.4–20.4 %, respectively. The respiratory quinone was ubiquinone-8. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminophospholipid. The predominant cellular fatty acids were summed feature 9 (10-methyl-C/iso-C  9, 22.2 %), iso-C (16.1 %) and iso-C (13.1 %). Based on the results of phylogenetic analysis, genome relatedness, and the physiological and chemotaxonomic properties of the isolate, strain IM 1326 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed (type strain IM 1326=CGMCC 1.17056=JCM 34227).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aliidiomarina , haloalkaliphilic bacterium , polyphasic taxonomy , soda lake and whole-genome sequence
Funding
This study was supported by the:
  • Science and Technology Basic Resources Investigation Project (Award 2017FY100300)
    • Principle Award Recipient: NotApplicable
  • Major Research Plan (Award 91751201)
    • Principle Award Recipient: HuaXiang
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-03-04
2024-12-09
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References

  1. Jean WD, Huang S-P, Chen J-S, Shieh WY. Kangiella taiwanensis sp. nov. and Kangiella marina sp. nov., marine bacteria isolated from shallow coastal water. Int J Syst Evol Microbiol 2012; 62:2229–2234 [View Article]
     [Google Scholar]
  2. Taborda M, Antunes A, Tiago I, Veríssimo A, Nobre MF et al. Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina. Syst Appl Microbiol 2009; 32:371–378 [View Article] [PubMed]
     [Google Scholar]
  3. Liu Y, Lai Q, Shao Z. Genome-based analysis reveals the taxonomy and diversity of the family Idiomarinaceae. Front Microbiol 2018; 9:2543 [View Article] [PubMed]
     [Google Scholar]
  4. Srinivas TNR, Anil Kumar P. Aliidiomarina haloalkalitolerans sp. nov., a marine bacterium isolated from coastal surface seawater. Antonie van Leeuwenhoek 2012; 101:761–768 [View Article] [PubMed]
     [Google Scholar]
  5. Chiu HH, Rogozin DY, Huang SP, Degermendzhy AG, Shieh WY et al. Aliidiomarina shirensis sp. nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina. Int J Syst Evol Microbiol 2014; 64:1334–1339 [View Article] [PubMed]
     [Google Scholar]
  6. Farooqui SM, Wright MH, Greene AC. Aliidiomarina minuta sp. nov., a haloalkaliphilic bacterium that forms ultra-small cells under non-optimal conditions. Antonie van Leeuwenhoek 2016; 109:83–93 [View Article] [PubMed]
     [Google Scholar]
  7. Ali Amoozegar M, Shahinpei A, Abolhassan Shahzadeh Fazeli S, Schumann P, Spröer C et al. Aliidiomarina iranensis sp. nov., a haloalkaliphilic bacterium from a coastal-marine wetland. Int J Syst Evol Microbiol 2016; 66:2099–2105 [View Article] [PubMed]
     [Google Scholar]
  8. Wang G, Wu H, Zhang X, Zhang H, Yang X et al. Aliidiomarina sanyensis sp. nov., a hexabromocyclododecane assimilating bacterium from the pool of Spirulina platensis cultivation, Sanya, China. Antonie van Leeuwenhoek 2013; 104:309–314 [View Article] [PubMed]
     [Google Scholar]
  9. Xu L, Sun JQ, Wang LJ, Liu XZ, Ji YY et al. Aliidiomarina soli sp. nov., isolated from saline-alkaline soil. Int J Syst Evol Microbiol 2017; 67:724–728 [View Article] [PubMed]
     [Google Scholar]
  10. Zhang YJ, Zhang XY, Zhao HL, Zhou MY, Li HJ et al. Idiomarina maris sp. nov., a marine bacterium isolated from sediment. Int J Syst Evol Microbiol 2012; 62:370–375 [View Article] [PubMed]
     [Google Scholar]
  11. Wang X-T, Han J-R, Zheng W-S, Zhang X-K, Du Z-J. Aliidiomarina celeris sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2019; 69:171–176 [View Article] [PubMed]
     [Google Scholar]
  12. Shahinpei A, Amoozegar MA, Shahzadeh Fazeli SA, Schumann P, Spröer C et al. Aliidiomarina sedimenti sp. nov., a haloalkaliphilic bacterium in the family Idiomarinaceae. Int J Syst Evol Microbiol 2017; 67:2087–2092 [View Article] [PubMed]
     [Google Scholar]
  13. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article] [PubMed]
     [Google Scholar]
  14. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
     [Google Scholar]
  15. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
     [Google Scholar]
  16. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article] [PubMed]
     [Google Scholar]
  17. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
     [Google Scholar]
  18. Chaumeil PA, Mussig AJ, Hugenholtz P, Parks DH. GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database. Bioinformatics 2019; 36:1925–1927 [View Article] [PubMed]
     [Google Scholar]
  19. Ventosa A, Quesada E, Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A. numerical taxonomy of moderately halophilic Gram-negative rods. Microbiology 1982; 128:1959–1968 [View Article]
     [Google Scholar]
  20. Dussault HP. An improved technique for staining red halophilic bacteria. J Bacteriol 1955; 70:484–485 [View Article] [PubMed]
     [Google Scholar]
  21. Harrigan JT, Langer A, Hung CT, Pelosi MA, Washington E. Predictive value of human placental lactogen determinations in pregnancy. Obstet Gynecol 1976; 47:443–445 [PubMed]
     [Google Scholar]
  22. Mata JA, Martínez-Cánovas J, Quesada E, Béjar V. A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 2002; 25:360–375 [View Article] [PubMed]
     [Google Scholar]
  23. Cui HL, Lin ZY, Dong Y, Zhou PJ, Liu SJ. Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 2007; 57:2204–2206 [View Article] [PubMed]
     [Google Scholar]
  24. Oren A, Ventosa A, Grant WD. Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 1997; 47:233–238 [View Article]
     [Google Scholar]
  25. Biswas S, Chowdhury B, Ray BC. A novel spectrofluorimetric method for the ultra trace analysis of nitrite and nitrate in aqueous medium and its application to air, water, soil and forensic samples. Talanta 2004; 64:308–312 [View Article] [PubMed]
     [Google Scholar]
  26. Cui HL, Gao X, Yang X, Xu XW. Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. Extremophiles 2010; 14:493–499 [View Article] [PubMed]
     [Google Scholar]
  27. Collins MD. Isoprenoid quinone analyses in bacterial classification and identification. In Chemical Methods in Bacterial Systematics London: Academic Press; 1985
     [Google Scholar]
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Aliidiomarina halalkaliphila sp. nov., a haloalkaliphilic bacterium isolated from a soda lake in Inner Mongolia Autonomous Region, China
Int J Syst Evol Microbiol 72, 005263 (2022); https://doi.org/10.1099/ijsem.0.005263
/content/journal/ijsem/10.1099/ijsem.0.005263
/content/journal/ijsem/10.1099/ijsem.0.005263
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