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Volume 72, Issue 3

sp. nov., a haloalkaliphilic bacterium isolated from a soda lake in Inner Mongolia Autonomous Region, China Open Access

Abstract

A haloalkaliphilic strain (IM 1326) was isolated from brine sampled at a soda lake in the Inner Mongolia Autonomous Region, China. Cells of the strain were rod-shaped and motile. Strain IM 1326 was able to grow at 4–42 °C (optimum, 37 °C) with 0–13.0 % (w/v) NaCl concentrations (optimum at 4.0–6.0 %) and at pH 7.5–11.0 (optimum at 9.0–10.0). The 16S rRNA gene phylogenetic analysis revealed that the isolate belongs to the genus and is closely related to the type strains of (95.8 % sequence similarity), (95.7 %), (95.4 %) and (95.3 %). The whole genome of strain IM 1326 was sequenced, and the genomic DNA G+C content was 49.7 mol%. Average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between the isolate and the related species were 68.1–84.9 %, 76–78 % and 18.4–20.4 %, respectively. The respiratory quinone was ubiquinone-8. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminophospholipid. The predominant cellular fatty acids were summed feature 9 (10-methyl-C/iso-C  9, 22.2 %), iso-C (16.1 %) and iso-C (13.1 %). Based on the results of phylogenetic analysis, genome relatedness, and the physiological and chemotaxonomic properties of the isolate, strain IM 1326 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed (type strain IM 1326=CGMCC 1.17056=JCM 34227).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aliidiomarina , haloalkaliphilic bacterium , polyphasic taxonomy , soda lake and whole-genome sequence
Funding
This study was supported by the:
  • Science and Technology Basic Resources Investigation Project (Award 2017FY100300)
    • Principle Award Recipient: NotApplicable
  • Major Research Plan (Award 91751201)
    • Principle Award Recipient: HuaXiang
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-03-04
2024-04-25
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Aliidiomarina halalkaliphila sp. nov., a haloalkaliphilic bacterium isolated from a soda lake in Inner Mongolia Autonomous Region, China
Int J Syst Evol Microbiol 72, 005263 (2022); https://doi.org/10.1099/ijsem.0.005263
/content/journal/ijsem/10.1099/ijsem.0.005263
/content/journal/ijsem/10.1099/ijsem.0.005263
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