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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 2

sp. nov., a novel CO-reducing acetogenic bacterium isolated from peatland soil No Access

Abstract

Some species of the genus are efficient acetate producers and have been deemed useful for upgrading industrial biogas. An acetogenic, strictly anaerobic, Gram-stain-positive, subterminal endospore-forming bacterium designated strain PL3 was isolated from peatland soil enrichments with H and CO. Cells of strain PL3 were 0.8–1.0×4.0–10.0 µm in size and rod-shaped. Growth of strain PL3 occurred at pH 6.0–7.5 (optimum, pH 7.0), at 20–40 °C (optimum, 30 °C) and with 0–1.5 % (w/v) NaCl (optimum, 0.5%). Biochemical analyses revealed that strain PL3 metabolized lactose, maltose, raffinose, rhamnose, lactic acid, sorbitol, arabinose and glycerol. Acetic acid was the predominant metabolite under anaerobic respiration with H/CO. The major cellular fatty acids were C, C 9 and C cyc. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, aminolipid and aminophospholipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PL3 belongs to the genus with the highest sequence similarity to DSM 17425 (98.6 %) followed by (97.8 %). The genomic DNA G+C content of strain PL3 was 31.1 mol%.The genomic DNA–DNA hybridization value between strain PL3 and DSM 17425 was 25.1 %, with an average nucleotide identity of 80.2 %. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain PL3 was suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PL3 (=DSM 111812=TISTR 2984).

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  • Accepted:
  • Published Online:
Keyword(s): acetogenic bacteria , biogas upgrading , Clostridium , CO2-reducing bacteria and peatland soil
Funding
This study was supported by the:
  • Royal Golden Jubilee (RGJ) Ph.D. Programme (Award (PHD/0230/2560))
    • Principle Award Recipient: SupattraIn-chan
  • Senior Research Scholar (Award RTA6280001)
    • Principle Award Recipient: AlissaraReungsang
  • Talanted Mid-Career Research Grant (Award N41A640088)
    • Principle Award Recipient: sompongo-thong
  • Research and Researcher for Industry Grant (Award RRI_PHD58I0053)
    • Principle Award Recipient: WisarutTukanghan
  • Royal Golden Jubilee (RGJ) Ph.D. Programme (Award (PHD/0108/2558))
    • Principle Award Recipient: SrisudaChaikitkaew
© 2022 The Authors
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2022-02-21
2024-04-18
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Clostridium thailandense sp. nov., a novel CO2-reducing acetogenic bacterium isolated from peatland soil
Int J Syst Evol Microbiol 72, 005254 (2022); https://doi.org/10.1099/ijsem.0.005254
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