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Volume 72, Issue 2

sp. nov., isolated from soil No Access

Abstract

A Gram-stain-variable, motile, aerobic, spore-forming, rod-shaped bacterium, designated as strain LOB 377, was isolated from soil sampled in Koka County (now Konan City), in Shiga Prefecture, Japan. To determine its taxonomic position, the bacterium was evaluated by a polyphasic approach based on genomic, phenotypic and chemotaxonomic tests. From phylogenetic analysis based on 16S rRNA gene sequences, strain LOB 377 (LC570960) was revealed to have the highest similarity to the type strain of FJAT-13985 (9.1 %). The average nucleotide identity and digital DNA–DNA hybridization values between both strains based on whole genome sequences were 84.1 and 42.8 %, respectively, which were below the recommended thresholds. The strain grew at 15–45 °C (optimum, 25–37 °C), at pH 5.5–9.5 (optimum, pH 6.5–8.5) and with 1.0–2.0 % (w/v) NaCl. Cell-wall peptidoglycan of the strain contained -diaminopimelic acid. The major menaquinone was menaquinone-7. Predominant fatty acids were iso-C, anteiso-C, iso-C and C ω11. The polar lipids were phosphatidylglycerol, unidentified phospholipid and unidentified aminophospholipid. The DNA G+C content was 40.5 mol%. According to the genomic, phenotypic and chemotaxonomic results, strain LOB 377 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is LOB 377 (=ATCC 31382=NBRC 114637=DSM 113418).

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Keyword(s): Firmicutes , LOB 377T , Neobacillus and novel species
© 2022 The Authors
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2022-02-22
2024-04-19
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Neobacillus kokaensis sp. nov., isolated from soil
Int J Syst Evol Microbiol 72, 005253 (2022); https://doi.org/10.1099/ijsem.0.005253
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