1887

Abstract

A Gram-stain-negative, heterotrophic, aerobic, non-motile, rod-shaped bacterial strain (GW1-59) belonging to the genus was isolated from coastal sediment collected from the Chinese Great Wall Station, Antarctica. The strain was identified using a polyphasic taxonomic approach. The strain grew well on Reasoner's 2A media and could grow in the presence of 0–4 % (w/v) NaCl (optimum, 1 %), at pH 9.0–11.0 and at 15–37 °C (optimum, 30 °C). Strain GW1-59 possessed ubiquinone-8 as the sole respiratory quinone. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were summed feature 9 (10-methyl C and/or iso-C 9), iso-C, iso-C, iso-C, C and iso-C 3-OH. DNA–DNA relatedness with Ko07, the nearest phylogenetic relative (98.5 % 16S rRNA gene sequence similarity) was 23.4 % (21.1–25.9 %). The average nucleotide identity value between strain GW1-59 and Ko07 was 80.1 %. The physiological and biochemical results and low level of DNA–DNA relatedness suggested the phenotypic and genotypic differentiation of strain GW1-59 from other species. On the basis of phenotypic, phylogenetic and genotypic data, a novel species, sp. nov., is proposed. The type strain is GW1-59 (=CCTCC AB 2019390=KCTC 72831).

Funding
This study was supported by the:
  • the National Key R&D Program of China (Award 2018YFC1406705)
    • Principle Award Recipient: JingLi
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2022-02-16
2022-05-18
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