1887

Abstract

Two new marine actinobacteria, designated as J2-1 and J2-2, were isolated from a coral, , collected from Rayong Province, Thailand. The taxonomic positions of the two strains were identified based on polyphasic taxonomy. Based on morphological characteristics and chemotaxonomy, strains J2-1 and J2-2 were identified as members of the genus and , respectively. Strains J2-1 and J2-2 showed the highest 16S rRNA gene sequence similarity to T44 (98.62 %) and VN05A0415 (98.08 %), respectively. Strain J2-1 had chemotaxonomic properties resembling members of the genus . -Diaminopimelic acid, glucose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside, unidentified aminolipid and five unidentified phospholipids were detected as the polar lipids. The major cellular fatty acids were C iso, C anteiso, C iso, C, C anteiso, C iso and C iso. Strain J2-2 a showed similar cell composition to members of the genus . Both isomers of - and -diaminopimelic acid were detected in the peptidoglycan. Arabinose, galactose, madurose and xylose were observed in the whole-cell hydrolysate. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylcholine, an unidentified phospholipid and an unidentified glycolipid. The major cellular fatty acids were C, C ω9, C 10-methyl, tuberculostearic acid, C and C. Both strains could be distinguished from their closely related type strains according to their phenotypic characteristics. Comparative genome analysis indicated the delineation of two novel species based on digital DNA–DNA hybridization and average nucleotide identity values, which were below 70 and 95 %, respectively. The names proposed are sp. nov. (J2-1=TBRC 13503=NBRC 115066) and sp. nov. (J2-2=TBRC 13504=NBRC 114885).

Funding
This study was supported by the:
  • Faculty of Pharmaceutical Sciences, Chulalongkorn University (Award (Phar2564-RG004))
    • Principle Award Recipient: WongsakornPhongsopitanun
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2022-02-21
2024-07-25
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