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Volume 72, Issue 2

gen. nov., sp. nov., a member of a novel genus of the family , isolated from the gut of the plastic-eating mealworm L. No Access

Abstract

A Gram-negative, white-pigmented, motile and rod-shaped strain, BIT-L3, was isolated from the gut of plastic-eating mealworm L. Its taxonomic position was determined by using a polyphasic approach. A preliminary analysis based on the 16S rRNA gene sequence (1445 bp) revealed that this strain was closely related to the members within the family . Phylogenetic trees based on the concatenated partial sequences of seven housekeeping genes (, , , , , , ) and genome sequences further showed that strain BIT-L3 constituted a separate lineage within the family DNA–DNA hybridization values and average nucleotide identity values between strain BIT-L3 and its closest related species within the family were less than 21.8 and 76.7 %, respectively. The major fatty acids (>5 %) of strain BIT-L3 were C, C, C cyclo, summed feature 8 (comprising C ω7 and/or C ω6), summed feature 3 (comprising C ω7 and/or C ω6 and/or iso-C 2-OH) and summed feature 2 (comprising iso-C I/C 3-OH and/or C aldehyde and/or an unknown fatty acid of equivalent chain length 10.9525). Its genomic DNA G+C content was 53.7 mol%. Based on the results of phylogenetic, physiological and biochemical analyses, strain BIT-L3 is considered to represent a novel species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is BIT-L3 (=CCTCC AB 2020371=LMG 32222=TBRC 14825).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Enterobacteriaceae , BIT-L3T and Tenebrionibacter intestinalis
Funding
This study was supported by the:
  • the Fundamental Research Funds for the Central Universities
    • Principle Award Recipient: YuYang
  • the Beijing Institute of Technology Research Fund Program for Young Scholars (Award No. 3160011181804)
    • Principle Award Recipient: YuYang
  • Young Elite Scientist Sponsorship Program of the China Association of Science and Technology (Award No. 2017QNRC001)
    • Principle Award Recipient: YuYang
  • National Natural Science Foundation of China (Award No. 51603004)
    • Principle Award Recipient: YuYang
  • National Natural Science Foundation of China (Award No. 31961133015)
    • Principle Award Recipient: YuYang
© 2022 The Authors
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2022-02-14
2024-04-20
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Tenebrionibacter intestinalis gen. nov., sp. nov., a member of a novel genus of the family Enterobacteriaceae, isolated from the gut of the plastic-eating mealworm Tenebrio molitor L.
Int J Syst Evol Microbiol 72, 005246 (2022); https://doi.org/10.1099/ijsem.0.005246
/content/journal/ijsem/10.1099/ijsem.0.005246
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