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Abstract
A Gram-stain-positive, aerobic, motile, rod-shaped bacterium, designated strain LAM9210T, was isolated from a saline soil sample collected from Lingxian County, Shandong Province, PR China. Analysis of the 16S rRNA gene sequence of the isolate revealed highest sequence similarities to the type strain of Sporosarcina pasteurii NCIMB 8841T (97.6 % sequence similarity). The genomic G+C content was 40.4 mol%. The average nucleotide identity and in silico DNA–DNA hybridization values between strain LAM9210T and the type strain of the most closely related species S. pasteurii NCIMB 8841T were 73.6 and 20.6 %, respectively. Strain LAM9210T was found to grow at 10–40 °C (optimum, 30 °C), at pH 6.0–10.0 (optimum, pH 9.0) and with 0–6 % (w/v) NaCl (optimum, 0.5 %), respectively. The major fatty acids were anteiso-C15 : 0 and iso-C14 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified phospholipid. Menaquinone-7 was detected as the predorminant respiratory quinone. Strain LAM9210T contained glycine, lysine, alanine and glutamic acid as the diagnostic amino acids in the cell-wall peptidoglycan. On the basis of phenotypic, phylogenetic and genotypic data, strain LAM9210T is considered to represent a novel species of the genus Sporosarcina , for which the name Sporosarcina jiandibaonis sp. nov. is proposed. The type strain is LAM9210T (=CGMCC 1.18607T=GDMCC 1.2002T=JCM 32514T).
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Funding
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Western Light Project of Chinese Academy of Science
(Award 2019-FPGGRC-002)
- Principle Award Recipient: YanGao
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National Key R&D Program of China
(Award 2017YFD0800702)
- Principle Award Recipient: ZhiyongRuan
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special project for basic scientific activities of non-profit institutes supported the government of Xinjiang Uyghur Autonomous Region
(Award KY2020105)
- Principle Award Recipient: ZhiyongRuan
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Science and Technology Planning Project of Shenzhen Municipality
(Award 2020A01001-2)
- Principle Award Recipient: ZhiyongRuan
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Open Funds of Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin
(Award Tarim University, BRZD1603)
- Principle Award Recipient: ZhiyongRuan
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National Natural Science Foundation of China
(Award 31670006)
- Principle Award Recipient: ZhiyongRuan
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National Natural Science Foundation of China
(Award NSFC 32070004)
- Principle Award Recipient: ZhiyongRuan