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Volume 72, Issue 2

sp. nov., an indigo-reducing facultative anaerobic alkaliphile isolated from indigo fermentation liquor used for dyeing No Access

Abstract

Two facultative anaerobic and facultative alkaliphilic indigo-reducing strains, designated F-1 and F-2, were isolated from indigo fermentation liquor produced from couched woad fermentation-based Indian indigo fermentation fluid. The 16S rRNA gene phylogeny showed that WS4937 (99.5%) was the closest neighbour of F-1. The isolated bacterial cells were Gram-stain-positive and facultative anaerobic coccoids. Strain F-1 grew at between 5 and 37 °C with optimum growth between 28‒32 °C. The isolate grew in a pH range of 7.0‒10.5, with optimum growth between pH 9.0‒10.5. The DNA G+C content was 37.6 mol% (HPLC). The whole-cell fatty acid profile mainly consisted (>10 %) of C, C ω9, C and C ω9. The digital DNA–DNA hybridization value between strain F-1 and WS4937 was 52.9 %. Based on their physiological and biochemical characteristics, and phylogenetic and genomic data, the isolates can be discriminated from WS4937. The name sp. nov. is proposed. The type strain of this species is F-1 (JCM 34140=NCIMB 15255).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): alkaliphilic , Fundicoccus fermenti , indigo fermentation liquor and indigo reduction
Funding
This study was supported by the:
  • Institute for Fermentation, Osaka (Award G-2020-3-035)
    • Principle Award Recipient: IsaoYumoto
© 2022 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005239
2022-02-14
2024-04-18
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Fundicoccus fermenti sp. nov., an indigo-reducing facultative anaerobic alkaliphile isolated from indigo fermentation liquor used for dyeing
Int J Syst Evol Microbiol 72, 005239 (2022); https://doi.org/10.1099/ijsem.0.005239
/content/journal/ijsem/10.1099/ijsem.0.005239
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