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Volume 72, Issue 1

sp. nov., an extremely halophilic archaeon isolated from a saturated brine pond of a saltern No Access

Abstract

A novel extremely halophilic archaeon, strain RHB-C, was isolated from a saturated brine pond of a solar saltern in Bolinao, Pangasinan, Philippines. Colonies were orange-red-pigmented, smooth, convex and round on a solid modified growth medium containing 25 % (w/v) of total salts. Cells of strain RHB-C on the solid modified growth medium were ovoid-shaped (0.89–2.66 µm long), while the cells in a liquid modified growth medium were rod-shaped (1.53–5.65 µm long and 0.45–1.03 µm wide). The strain was Gram-stain-negative, motile and strictly aerobic. Strain RHB-C grew with NaCl concentrations ranging from 10 to 30 % (w/v; optimum, 20–25 %), at pH 6.5–8.5 (optimum, pH 7.0–7.5) and at 20–55 °C (optimum, 40–45 °C). Furthermore, the strain grew even in the absence of Mg; however, when supplemented with Mg, growth was observed optimally at 0.2–0.4 M Mg. The 16S rRNA gene phylogeny inferred that the strain is a member of the genus and was related to CGMCC 1.3527 (99.0 %), DSM 3755 (98.8 %), Ch2 (98.8 %), NRC 34021 (98.4 %) and JCM 9100 (98.1 %). The ′ gene sequences also showed that strain RHB-C is related to JCM 12388 (97.1 %), JCM 9100 (97.1 %), JCM 9275 (96.5 %), JCM 14715 (96.5 %), JCM 10477 (96.3%), JCM 8880 (96.2%) and DSM 14210 (95.6 %). The DNA G+C content of strain RHB-C was 68.7 mol% (genome). Digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain RHB-C and the closely related species of were below 40 and 90 %, respectively, which are far below the thresholds to delineate a new species. The polar lipids of strain RHB-C were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulphate and sulfated mannosyl glycosyl diether. Based on dDDH and ANI values, and the significant morphological and physiological differences from known taxa, it is hereby suggested that strain RHB-C represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RHB-C (=KCTC 4274=CMS 2103).

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Keyword(s): Haloarchaeon , polyphasic taxonomy and solar saltern
© 2022 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005231
2022-01-17
2024-04-24
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Halorubrum salinarum sp. nov., an extremely halophilic archaeon isolated from a saturated brine pond of a saltern
Int J Syst Evol Microbiol 72, 005231 (2022); https://doi.org/10.1099/ijsem.0.005231
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