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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 1

gen. nov., sp. nov., isolated from a culture and reclassification of as comb. nov. and as comb. nov Free

Abstract

A novel bacterial strain, NIBR3, was isolated from a culture. Strain NIBR3 was characterized as Gram-negative, rod-shaped, catalase- and oxidase-positive, and aerobic. The 16S rRNA gene sequence analysis showed that strain NIBR3 was most closely related to B2.3 (=KCTC 52461), B7 (=KCTC 42783) and CCBAU 33460 (=HAMBI 3277), at 98.7, 97.2 and 97.2% similarity, respectively. Our phylogenetic analyses revealed that three strains [strain NIBR3 with the previously reported two species ( B2.3 and B7)] formed a distinct cluster from other type strains. The average nucleotide identity of strain NIBR3 relative to B2.3 B7, and CCBAU 33460 was found to be 84.3, 79.4 and 75.8 %, with average amino-acid identities of 85.1, 74.8 and 64.3 %, and digital DNA–DNA hybridization values of 27.6, 22.6 and 20.7 %, respectively. The genome size and genomic DNA G+C content of NIBR3 were 6.1 Mbp and 67.9 mol%, respectively. Growth of strain NIBR3 was observed at 23–45 °C (optimum, 33 °C), at pH 6–11 (optimum, 8) and in the presence of 0–4 % (w/v) NaCl (optimum, 0 %). The major polar lipids in this novel strain were phosphatidylethanolamine, phosphatidylcholine and phosphatidylmethylethanolamine. The predominant respiratory quinone was Q-10. Summed feature 8 (C 7 and/or C ) was the most abundant cellular fatty acid in strain NIBR3. Based on genotypic characteristics using our genomic data, strain NIBR3 was identified as a member of new genus, gen. nov., with the two aforementioned stains. The type strain f the novel species, sp. nov., is NIBR3 (=KACC 22092=HAMBI 3738). We also reclassified and as comb. nov. and comb. nov., respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aquibium , freshwater , Microcystis aeruginosa , nitrate , Phyllobacteriaceae and ubiquinone Q10
Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR202002108)
    • Principle Award Recipient: WoojunPark
© 2022 The Authors
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2022-01-17
2025-04-22
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Aquibium microcysteis gen. nov., sp. nov., isolated from a Microcystis aeruginosa culture and reclassification of Mesorhizobium carbonis as Aquibium carbonis comb. nov. and Mesorhizobium oceanicum as Aquibium oceanicum comb. nov
Int J Syst Evol Microbiol 72, 005230 (2022); https://doi.org/10.1099/ijsem.0.005230
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