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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 2

sp. nov., a novel aromatic hydrocarbon-degrading bacterium isolated from benzene-, and xylene-degrading enrichment cultures, and emended description of the genus No Access

Abstract

Two Gram-reaction-negative strains, designated as B13 and MA2-2, were isolated from two different aromatic hydrocarbon-degrading enrichment cultures and characterized using a polyphasic approach to determine their taxonomic position. The two strains had identical 16S rRNA gene sequences and were most closely related to E9 (97.36 %) and SA-279 (96.33 %). Cells were facultatively aerobic rods and motile with a single polar flagellum. The strains were able to degrade ethylbenzene as sole source of carbon and energy. The assembled genome of strain B13 had a total length of 4.91 Mb and the DNA G+C content was 68.8 mol%. The predominant fatty acids (>5 % of the total) of strains B13 and MA2-2 were C 7/C 6, C 7/C 6 and C. The major ubiquinone of strain B13 was Q10, while the major polar lipids were phosphatidyl--methylethanolamine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and a phospholipid. Based on phenotypic characteristics and phylogenetic data, it is concluded that strains B13 and MA2-2 are members of the genus and represent a novel species for which the name sp. nov. is proposed. The type strain of the species is strain B13 (=LMG 32346=NCAIM B.02665).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): BTEX degradation , Hyphomicrobiales , new taxa and Pinisolibacter aquiterrae
Funding
This study was supported by the:
  • Tempus Public Foundation (Award Stipendium Hungaricum Scholarship Programme)
    • Principle Award Recipient: SinchanBanerjee
  • Hungarian Scientific Research Fund (Award FK 134439)
    • Principle Award Recipient: AndrásTáncsics
  • Ministry of Innovation and Technology of Hungary (Award TKP2020-IKA-12)
    • Principle Award Recipient: NotApplicable
© 2022 The Authors
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2022-02-09
2024-04-16
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Pinisolibacter aquiterrae sp. nov., a novel aromatic hydrocarbon-degrading bacterium isolated from benzene-, and xylene-degrading enrichment cultures, and emended description of the genus Pinisolibacter
Int J Syst Evol Microbiol 72, 005229 (2022); https://doi.org/10.1099/ijsem.0.005229
/content/journal/ijsem/10.1099/ijsem.0.005229
/content/journal/ijsem/10.1099/ijsem.0.005229
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