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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 2

sp. nov., an endophytic bacterium isolated from soybean ( L. cv. Gwangan) stems No Access

Abstract

A Gram-stain-negative, facultatively anaerobic, motile by gliding, rod-shaped, oxidase- and catalase-positive bacterial strain, designated BB8, was isolated from the stems of a Korean soybean cultivar ( L. cv. Gwangan). The strain produced a yellow pigment on tryptic soy agar. Growth of strain BB8 occurred at pH 5.0–8.0 (optimum, pH 7.0), at 10–35 °C (optimum, 25–30 °C) and in the presence of 0–1 % (w/v) NaCl (optimum, 0.5%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BB8 formed a lineage within the genus and was most closely related to SYP-B1015 (96.9 % 16S rRNA gene sequence similarity) and T13 (96.8%). The complete genome sequence of strain BB8 was 5 513 159 bp long with a G+C content of 34.1 mol%. The major fatty acids (>10 %) of strain BB8 were iso-C (21 %), summed feature 3 (comprising C ω7 and/or C ω6, 20.3%) and iso-C 3-OH (13.7%). The predominant polar lipids were phosphatidylethanolamine and unidentified aminolipids, and the major respiratory quinone was menaquinone-6. Based on these phenotypic, genotypic and chemotaxonomic characteristics, strain BB8 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BB8 (=KCTC 82167=CCTCC AB 2020070).

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Keyword(s): Flavobacterium endoglycinae sp. nov. , Glycine max and novel species
Funding
This study was supported by the:
  • Cooperative Research Program for Agriculture Science & Technology Development (Award PJ014052021)
    • Principle Award Recipient: Man-SooChoi
  • Basic Science Research Program through the National Research Foundation of Korea (Award NRF-2020R111A2072308)
    • Principle Award Recipient: JiyoungLee
  • Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (Award 321057051HD020)
    • Principle Award Recipient: JiyoungLee
© 2022 The Authors
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Flavobacterium endoglycinae sp. nov., an endophytic bacterium isolated from soybean (Glycine max L. cv. Gwangan) stems
Int J Syst Evol Microbiol 72, 005220 (2022); https://doi.org/10.1099/ijsem.0.005220
/content/journal/ijsem/10.1099/ijsem.0.005220
/content/journal/ijsem/10.1099/ijsem.0.005220
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