1887

Abstract

Two novel Gram-stain-negative, aerobic, rod-shaped, carotenoid-pigmented and non-flagellated bacteria, designated BC31-1-A7 and BC31-3-A3, were isolated from polyethylene-terephthalate-degrading bacterial consortia enriched from deep-sea sediment collected in the Pacific Ocean. Optimal growth of both strains was observed at 28–32 °C, at pH 7.5 and in the presence of 3–4% (w/v) NaCl. The 16S rRNA gene sequence analysis revealed that strains BC31-1-A7 and BC31-3-A3 were closely related to JCM 11811, KCTC 22173, DSM 13258, DSM 25030, JCM 33902 and KCTC 72200 with 96.8–98.9% sequence similarity. The 16S rRNA gene sequence similarity between strains BC31-1-A7 and BC31-3-A3 was 97.5%. The genomic G+C contents of strains BC31-1-A7 and BC31-3-A3 were 42.1 and 41.6 mol%, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strain BC31-3-A3, strain BC31-1-A7 and their six closely related type strains were 77.6–84.3% and 20.5–27.9%, respectively. Menaquinone-6 was detected as the major isoprenoid quinone in all strains. Their major fatty acids were iso-C, iso-C G and iso-C 3-OH. The major polar lipids of strains BC31-1-A7 and BC31-3-A3 were identified as one phosphatidylethanolamine, some unidentified polar lipids and one aminolipid. Based on their distinct taxonomic characteristics, strains BC31-1-A7 and BC31-3-A3 represent two novel species in the genus . The names proposed to accommodate these two strains are sp. nov. and sp. nov., and the type strains are BC31-1-A7 (=MCCC M23246=KCTC 82569) and BC31-3-A3 (=MCCC M23216=KCTC 82302), respectively.

Funding
This study was supported by the:
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award NIMR-2020-9)
    • Principle Award Recipient: ZongzeShao
  • Xiamen Ocean Economic Innovation and Development Demonstration Project (Award 16PZP001SF16)
    • Principle Award Recipient: ZongzeShao
  • COMRA program (Award No. DY135-B2-01)
    • Principle Award Recipient: ZongzeShao
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005217
2022-02-01
2024-03-28
Loading full text...

Full text loading...

References

  1. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North sea intertidal sediment. Int J Syst Evol Microbiol 2001; 51:1997–2006 [View Article]
    [Google Scholar]
  2. Yoon JH, Lee MH, Oh TK, Park YH. Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda. Int J Syst Evol Microbiol 2005; 55:1015–1019 [View Article]
    [Google Scholar]
  3. Hwang CY, Kim MH, Bae GD, Zhang GI, Kim YH et al. Muricauda olearia sp. nov., isolated from crude-oil-contaminated seawater, and emended description of the genus Muricauda. Int J Syst Evol Microbiol 2009; 59:1856–1861 [View Article] [PubMed]
    [Google Scholar]
  4. Oberbeckmann S, Osborn AM, Duhaime MB. Microbes on a bottle: substrate, season and geography influence community composition of microbes colonizing marine plastic debris. PLoS One 2016; 11:e0159289 [View Article] [PubMed]
    [Google Scholar]
  5. Denaro R, Aulenta F, Crisafi F, Di Pippo F, Cruz Viggi C et al. Marine hydrocarbon-degrading bacteria breakdown poly(ethylene terephthalate) (PET). Sci Total Environ 2020; 749:141608 [View Article] [PubMed]
    [Google Scholar]
  6. Liu L, Yu M, Zhou S, Fu T, Sun W et al. Muricauda alvinocaridis sp. nov., isolated from shrimp gill from the Okinawa Trough. Int J Syst Evol Microbiol 2020; 70:1666–1671 [View Article] [PubMed]
    [Google Scholar]
  7. Zhang X, Liu X, Lai Q, Du Y, Sun F et al. Muricauda indica sp. nov., isolated from deep sea water. Int J Syst Evol Microbiol 2018; 68:881–885 [View Article] [PubMed]
    [Google Scholar]
  8. Wu Y-H, Yu P-S, Zhou Y-D, Xu L, Wang C-S et al. Muricauda antarctica sp. nov., a marine member of the Flavobacteriaceae isolated from Antarctic seawater. Int J Syst Evol Microbiol 2013; 63:3451–3456 [View Article] [PubMed]
    [Google Scholar]
  9. Arun AB, Chen W-M, Lai W-A, Chao J-H, Rekha PD et al. Muricauda lutaonensis sp. nov., a moderate thermophile isolated from a coastal hot spring. Int J Syst Evol Microbiol 2009; 59:2738–2742 [View Article] [PubMed]
    [Google Scholar]
  10. Yoon JH, Kang SJ, Jung YT, Oh TK. Muricauda lutimaris sp. nov., isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 2008; 58:1603–1607 [View Article] [PubMed]
    [Google Scholar]
  11. Yang C, Li Y, Guo Q, Lai Q, Wei J et al. Muricauda zhangzhouensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2013; 63:2320–2325 [View Article] [PubMed]
    [Google Scholar]
  12. Vizzotto CS, Peixoto J, Green SJ, Lopes FAC, Ramada MHS et al. Muricauda brasiliensis sp. nov., isolated from a mat-forming cyanobacterial culture. Braz J Microbiol 2021; 52:325–333 [View Article] [PubMed]
    [Google Scholar]
  13. Zhang Z, Gao X, Qiao Y, Wang Y, Zhang XH. Muricauda pacifica sp. nov., isolated from seawater of the South Pacific Gyre. Int J Syst Evol Microbiol 2015; 65:4087–4092 [View Article] [PubMed]
    [Google Scholar]
  14. Dang Y-R, Sun Y-Y, Sun L-L, Yuan X-X, Li Y et al. Muricauda nanhaiensis sp. nov., isolated from seawater of the South China Sea. Int J Syst Evol Microbiol 2019; 69:2089–2094 [View Article] [PubMed]
    [Google Scholar]
  15. Liu SQ, Sun QL, Sun YY, Yu C, Sun L. Muricauda iocasae sp. nov., isolated from deep sea sediment of the South China Sea. Int J Syst Evol Microbiol 2018; 68:2538–2544 [View Article] [PubMed]
    [Google Scholar]
  16. Dyksterhouse SE, Gray JP, Herwig RP, Lara JC, Staley JT. Cycloclasticus pugetii gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium from marine sediments. Int J Syst Bacteriol 1995; 45:116–123 [View Article]
    [Google Scholar]
  17. Li G, Lai Q, Yan P, Shao Z. Roseovarius amoyensis sp. nov. and Muricauda amoyensis sp. nov., isolated from the Xiamen coast. Int J Syst Evol Microbiol 2019; 69:3100–3108 [View Article] [PubMed]
    [Google Scholar]
  18. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
    [Google Scholar]
  19. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article] [PubMed]
    [Google Scholar]
  20. Dong B, Zhu S, Chen T, Ren N, Chen X et al. Muricauda oceani sp. nov., isolated from the East Pacific Ocean. Int J Syst Evol Microbiol 2020; 70:3839–3844 [View Article] [PubMed]
    [Google Scholar]
  21. Guo LL, Wu D, Sun C, Cheng H, Xu XW et al. Muricauda maritima sp. nov., Muricauda aequoris sp. nov. and Muricauda oceanensis sp. nov., three marine bacteria isolated from seawater. Int J Syst Evol Microbiol 2020; 70:6240–6250
    [Google Scholar]
  22. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 2014; 30:2114–2120 [View Article] [PubMed]
    [Google Scholar]
  23. Li J, Qi M, Lai Q, Wang G, Shao Z. Sunxiuqinia indica sp. nov., isolated from deep sea. Int J Syst Evol Microbiol 2020; 70:4186–4192 [View Article] [PubMed]
    [Google Scholar]
  24. Na S-I, Kim YO, Yoon S-H, Ha S-M, Baek I et al. UBCG: Up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 2018; 56:280–285 [View Article] [PubMed]
    [Google Scholar]
  25. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  26. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article] [PubMed]
    [Google Scholar]
  27. Zhou G, Dudgeon C, Li M, Cao Y, Zhang L et al. Molecular cloning of the HGD gene and association of SNPs with meat quality traits in Chinese red cattle. Mol Biol Rep 2010; 37:603–611 [View Article] [PubMed]
    [Google Scholar]
  28. Zhu S, Lu Y, Xu X, Chen J, Yang J et al. Isolation and identification of a gene encoding 4-hydroxyphenylpyruvate dioxygenase from the red-brown pigment-producing bacterium Alteromonas stellipolaris LMG 21856. Folia Microbiol 2015; 60:309–316 [View Article] [PubMed]
    [Google Scholar]
  29. Zhang Y, Colabroy KL, Begley TP, Ealick SE. Structural studies on 3-hydroxyanthranilate-3,4-dioxygenase: the catalytic mechanism of a complex oxidation involved in NAD biosynthesis. Biochemistry 2005; 44:7632–7643 [View Article] [PubMed]
    [Google Scholar]
  30. Sasser M. In Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  31. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  32. Ross HNM, Collins MD, Tindall BJ, Grant WD. A rapid procedure for the detection of archaebacterial lipids in halophilic bacteria. Microbiology 1981; 123:75–80 [View Article]
    [Google Scholar]
  33. Collins M. Isoprenoid quinone analyses in bacterial classification and identification. Soc Appl Bacteriol Tech Ser 1985; 20:267–287
    [Google Scholar]
  34. Wang Y, Yang X, Liu J, Wu Y, Zhang XH. Muricauda lutea sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2017; 67:1064–1069 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005217
Loading
/content/journal/ijsem/10.1099/ijsem.0.005217
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error