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Abstract

Two novel Gram-stain-negative, aerobic, rod-shaped, carotenoid-pigmented and non-flagellated bacteria, designated BC31-1-A7 and BC31-3-A3, were isolated from polyethylene-terephthalate-degrading bacterial consortia enriched from deep-sea sediment collected in the Pacific Ocean. Optimal growth of both strains was observed at 28–32 °C, at pH 7.5 and in the presence of 3–4% (w/v) NaCl. The 16S rRNA gene sequence analysis revealed that strains BC31-1-A7 and BC31-3-A3 were closely related to JCM 11811, KCTC 22173, DSM 13258, DSM 25030, JCM 33902 and KCTC 72200 with 96.8–98.9% sequence similarity. The 16S rRNA gene sequence similarity between strains BC31-1-A7 and BC31-3-A3 was 97.5%. The genomic G+C contents of strains BC31-1-A7 and BC31-3-A3 were 42.1 and 41.6 mol%, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strain BC31-3-A3, strain BC31-1-A7 and their six closely related type strains were 77.6–84.3% and 20.5–27.9%, respectively. Menaquinone-6 was detected as the major isoprenoid quinone in all strains. Their major fatty acids were iso-C, iso-C G and iso-C 3-OH. The major polar lipids of strains BC31-1-A7 and BC31-3-A3 were identified as one phosphatidylethanolamine, some unidentified polar lipids and one aminolipid. Based on their distinct taxonomic characteristics, strains BC31-1-A7 and BC31-3-A3 represent two novel species in the genus . The names proposed to accommodate these two strains are sp. nov. and sp. nov., and the type strains are BC31-1-A7 (=MCCC M23246=KCTC 82569) and BC31-3-A3 (=MCCC M23216=KCTC 82302), respectively.

Funding
This study was supported by the:
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award NIMR-2020-9)
    • Principle Award Recipient: ZongzeShao
  • Xiamen Ocean Economic Innovation and Development Demonstration Project (Award 16PZP001SF16)
    • Principle Award Recipient: ZongzeShao
  • COMRA program (Award No. DY135-B2-01)
    • Principle Award Recipient: ZongzeShao
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2022-02-01
2022-07-06
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