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Volume 72, Issue 1

sp. nov., a nitrogen-fixing species isolated from the rhizosphere soil of a peach tree No Access

Abstract

A nitrogen-fixing, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 81-11, was isolated from rhizosphere soil of a peach tree collected from Handan, Hebei, PR China. From the comparison of 16S rRNA gene sequence, the strain is most closely related to DSM 27463 (96.9 %) and DSM 23593 (96.7 %). The genome size of strain 81-11 was 4.4 Mb, comprising 4879 predicted genes with a DNA G+C content of 50.0 mol%. The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species DSM 27463 and DSM 23593 were 71.8 and 72.1 %, respectively. The major cellular fatty acids were anteiso-C(47.8 %), iso-C (15.5 %) and iso-C (13.0 %). Menaquinone-7 was the major respiratory quinone. The polar lipids contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid, aminoglycopid, unknown polar lipids and unidentified aminophosphoglycolipid. Based on phylogenetic, genomic and phenotypic characteristics, strain 81-11 was classified as a novel species within the genus , for which the name sp. nov. is proposed. The type strain of is 81-11 (=JCM 34618=CGMCC 1.18907).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16SrRNA phylogeny , nitrogen-fixing bacteria , Paenibacillus caui and rhizospheres
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31870004)
    • Principle Award Recipient: MiaoGao
  • National Natural Science Foundation of China (Award 32000048)
    • Principle Award Recipient: QinLi
  • National Key Research and Development Program of China (Award 2019YFA0904700)
    • Principle Award Recipient: San-fengChen
© 2022 The Authors
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2022-01-31
2024-04-19
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Paenibacillus caui sp. nov., a nitrogen-fixing species isolated from the rhizosphere soil of a peach tree
Int J Syst Evol Microbiol 72, 005216 (2022); https://doi.org/10.1099/ijsem.0.005216
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