1887

Abstract

Novel thermophilic heterotrophic bacteria were isolated from the subsurface of the volcanic island Surtsey off the south coast of Iceland. The strains were isolated from tephra core and borehole fluid samples collected below 70 m depth. The Gram-negative bacteria were rod-shaped (0.3–0.4 µm wide, 1.5–7 µm long), aerobic, non-sporulating and non-motile. Optimal growth was observed at 70 °C, at pH 7–7.5 and with 1% NaCl. Phylogenetic analysis identified the strains as members of the genus . The type strain, ISCAR-7401, was genetically distinct from its closest relatives DSM 4252 and PRI 2902 based on 16S rRNA gene sequence similarity (95.81 and 96.01%, respectively), genomic average nucleotide identity (73.73 and 72.61%, respectively) and digital DNA–DNA hybridization (17.6 and 16.9%, respectively). The major fatty acids of ISCAR-7401 were iso-C, anteiso-C, anteiso-C and iso-C (>10 %). The major isoprenoid quinone was MK-7 while phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminophospholipid and a phospholipid were the predominant polar lipid components. Based on comparative chemotaxonomic, genomic and phylogenetic analyses, we propose that the isolated strain represents a novel species of the genus with the name sp. nov. The type strain is ISCAR-7401 (=DSM 112103=CIP 111906).

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2022-01-24
2022-07-05
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References

  1. Bergsten P, Vannier P, Mougeolle A, Rigaud L, Marteinsson VT. Rhodothermus bifroesti sp. nov., a thermophilic bacterium isolated from the basaltic subsurface of the volcanic island surtsey. Figshare 2022 [View Article]
    [Google Scholar]
  2. Cavalier-Smith T, Chao EEY. Multidomain ribosomal protein trees and the planctobacterial origin of neomura (eukaryotes, archaebacteria). Protoplasma 2020; 257:621–753 [View Article] [PubMed]
    [Google Scholar]
  3. Munoz R, Rosselló-Móra R, Amann R. Revised phylogeny of Bacteroidetes and proposal of sixteen new taxa and two new combinations including Rhodothermaeota phyl. nov. Syst Appl Microbiol 2016; 39:281–296 [View Article] [PubMed]
    [Google Scholar]
  4. Park MJ, Oh JH, Yang SH, Kwon KK. Roseithermus sacchariphilus gen. nov., sp. nov. and proposal of Salisaetaceae fam. nov., representing new family in the order Rhodothermales. Int J Syst Evol Microbiol 2019; 69:1213–1219 [View Article]
    [Google Scholar]
  5. Ludwig W. Family I. Rhodothermaceae fam. nov. In Bergey’s Manual of Systematics of Archaea and Bacteria Wiley; 2015 [View Article]
    [Google Scholar]
  6. Park S, Akira Y, Kogure K. The family Rhodothermaceae. In The Prokaryotes: Other Major Lineages of Bacteria and The Archaea Springer; 2014 pp 849–856 [View Article]
    [Google Scholar]
  7. Björnsdóttir SH, Pétursdóttir SK, Gudmundsdóttir EE, Olgudóttir E, Stefansson SK et al. Rhodocaloribacter litoris gen. nov., sp. nov., isolated from an intertidal hot spring. Int J Syst Evol Microbiol 2021; 71:005073 [View Article]
    [Google Scholar]
  8. Alfredsson GA, Kristjansson JK, Hjorleifsdottir S, Stetter KO. Rhodothermus marinus, gen. nov., sp. nov., a thermophilic, halophilic bacterium from submarine hot springs in Iceland. Microbiology 1988; 134:299–306 [View Article]
    [Google Scholar]
  9. Nunes OC, Donato MM, Da Costa MS. Isolation and characterization of Rhodothermus strains from S. Miguel, Azores. Syst Appl Microbiol 1992; 15:92–97 [View Article]
    [Google Scholar]
  10. Moreira L, Fernanda Nobre M, Sa-Correia I, Da Costa MS. Genomic typing and fatty acid composition of Rhodothermus marinus. Syst Appl Microbiol 1996; 19:83–90 [View Article]
    [Google Scholar]
  11. Petursdottir SK, Hreggvidsson GO, Da Costa MS, Kristjansson JK. Genetic diversity analysis of Rhodothermus reflects geographical origin of the isolates. Extremophiles 2000; 4:267–274 [View Article] [PubMed]
    [Google Scholar]
  12. Sako Y, Takai K, Ishida Y, Uchida A, Katayama Y. Rhodothermus obamensis sp. nov., a modern lineage of extremely thermophilic marine bacteria. Int J Syst Bacteriol 1996; 46:1099–1104 [View Article] [PubMed]
    [Google Scholar]
  13. Silva Z, Horta C, da Costa MS, Chung AP, Rainey FA. Polyphasic evidence for the reclassification of Rhodothermus obamensis Sako et al. 1996 as a member of the species Rhodothermus marinus Alfredsson et al. 1988. Int J Syst Evol Microbiol 2000; 50 Pt 4:1457–1461 [View Article] [PubMed]
    [Google Scholar]
  14. Marteinsson VT, Bjornsdottir SH, Bienvenu N, Kristjansson JK, Birrien JL. Rhodothermus profundi sp. nov., a thermophilic bacterium isolated from a deep-sea hydrothermal vent in the Pacific Ocean. Int J Syst Evol Microbiol 2010; 60:2729–2734 [View Article] [PubMed]
    [Google Scholar]
  15. Thorarinsson S, Þórarinsson S. The Surtsey eruption: course of events and the development of the new island. Surtsey Research Progress Report 1965; 1:51–55
    [Google Scholar]
  16. Jakobsson SP, Moore JG. The Surtsey research drilling project of 1979. Surtsey Research Progress Report 1982; 9:76–93
    [Google Scholar]
  17. Jakobsson SP, Thors K, Vésteinsson ÁT, Ásbjörnsdóttir L. Some aspects of the seafloor morphology at Surtsey volcano: the new multibeam bathymetric survey of 2007. Surtsey Research Surtsey Research Progress Report 2009; 12:9–20
    [Google Scholar]
  18. Weisenberger TB, Gudmundsson MT, Jackson MDG, Türke A, Kleine BI et al. Operational Report for the 2017 Surtsey Underwater Volcanic System for Thermophiles, Alteration Processes and INnovative Concretes (SUSTAIN) Drilling Project at Surtsey Volcano Iceland Potsdam: GFZ German Research Centre for Geosciences; 2019 p 240 [View Article]
    [Google Scholar]
  19. Jackson MD, Gudmundsson MT, Weisenberger TB, Rhodes JM, Stefánsson A et al. SUSTAIN drilling at surtsey volcano, iceland, tracks hydrothermal and microbiological interactions in basalt 50 years after eruption. Sci Dril 2019; 25:35–46 [View Article]
    [Google Scholar]
  20. Jackson MD, Gudmundsson MT, Bach W, Cappelletti P, Coleman NJ et al. Time-lapse characterization of hydrothermal seawater and microbial interactions with basaltic tephra at surtsey volcano. Sci Dril 2015; 20:51–58 [View Article]
    [Google Scholar]
  21. Bjornsdottir SH, Blondal T, Hreggvidsson GO, Eggertsson G, Petursdottir S et al. Rhodothermus marinus: physiology and molecular biology. Extremophiles 2006; 10:1–16 [View Article] [PubMed]
    [Google Scholar]
  22. Kristjansdottir T, Ron EYC, Molins-Delgado D, Fridjonsson OH, Turner C et al. Engineering the carotenoid biosynthetic pathway in Rhodothermus marinus for lycopene production. Metab Eng Commun 2020; 11:e00140 [View Article] [PubMed]
    [Google Scholar]
  23. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 2014; 30:2114–2120 [View Article] [PubMed]
    [Google Scholar]
  24. Nurk S, Bankevich A, Antipov D, Gurevich A, Korobeynikov A et al. Assembling genomes and mini-metagenomes from highly chimeric reads. In Deng M, Jiang R, Sun F, Zhang X. eds Research in Computational Molecular Biology Lecture Notes in Computer Science Berlin, Heidelberg: Springer; 2013 pp 158–170 [View Article]
    [Google Scholar]
  25. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article] [PubMed]
    [Google Scholar]
  26. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  27. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 2008; 9:75 [View Article] [PubMed]
    [Google Scholar]
  28. Arkin AP, Cottingham RW, Henry CS, Harris NL, Stevens RL et al. KBase: The United States Department of Energy Systems Biology Knowledgebase. Nat Biotechnol 2018; 36:566–569 [View Article] [PubMed]
    [Google Scholar]
  29. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article] [PubMed]
    [Google Scholar]
  30. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article] [PubMed]
    [Google Scholar]
  31. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article] [PubMed]
    [Google Scholar]
  32. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article] [PubMed]
    [Google Scholar]
  33. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
    [Google Scholar]
  34. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
    [Google Scholar]
  35. Hjorleifsdottir S, Skirnisdottir S, Hreggvidsson GO, Holst O, Kristjansson JK. Species composition of cultivated and noncultivated bacteria from short filaments in an Icelandic hot spring at 88 degrees C. Microb Ecol 2001; 42:117–125 [View Article] [PubMed]
    [Google Scholar]
  36. ZoBell CE. Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 1941; 4:41–75
    [Google Scholar]
  37. Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS. Methanogens: reevaluation of a unique biological group. Microbiol Rev 1979; 43:260–296 [View Article] [PubMed]
    [Google Scholar]
  38. Nunes OC, Donato MM, Manaia CM, Da Costa MS. The polar lipid and fatty acid composPolar Lipid and Fatty Acid Composition of Rhodothermus sStrains. Syst Appl Microbiol 1992; 15:59–62 [View Article]
    [Google Scholar]
  39. Tindall BJ. Lipid composition of Rhodothermus marinus. FEMS Microbiol Lett 1991; 80:65–68 [View Article]
    [Google Scholar]
  40. Simek R. Dictionary of Northern Mythology Cambridge: D.S. Brewer; 1993
    [Google Scholar]
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