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Volume 72, Issue 1

sp. nov., isolated from deep-sea sediment of the Mariana Trench No Access

Abstract

Two novel Gram-stain-negative, facultative anaerobic, non-flagellated, rod-shaped bacterial strains, designated MT13 and MT32, were isolated from sediment samples collected from the Mariana Trench at a depth of 8300 m. The two strains grew at −2–30 °C (optimum, 25 °C), at pH 5.5–10.0 (optimum, pH 7.5–8.0) and with 0–15 % (w/v) NaCl (optimum, 3–6 %). They did not reduce nitrate to nitrite nor hydrolyse Tweens 40 and 80, aesculin, casein, starch and DNA. The genomic G+C contents of draft genomes of strain MT13 and MT32 were 52.2 and 54.1 m ol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains MT13 and MT32 were affiliated with the genus , with the highest similarity to the type strain of . The values of average nucleotide identity and DNA–DNA hybridization between strain MT13 and MT32, and between strain MT13 and five closely related type strains of species indicated that strains MT13 and MT32 belonged to the same species, but represented a novel species in the genus of . The major cellular fatty acids of strains MT13 and MT32 were C, summed feature 3(C 7/6) and summed feature 8 (C 7/6). Major polar lipids of strains MT13 and MT32 included phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Ubiquinone-9 was the predominant respiratory quinone. Based on data from the present polyphasic study, strains MT13 and MT32 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MT13 (=MCCC 1K06389=KCTC 82923).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): deep-sea sediment , Halomonas profundi sp. nov. , Mariana Trench and polyphasic taxonomy
Funding
This study was supported by the:
  • Fundamental Research Funds for the Central Universities (Award 202141006)
    • Principle Award Recipient: PengWang
  • the Program of Shandong for Taishan Scholars (Award tspd20181203)
    • Principle Award Recipient: NotApplicable
  • Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (Award 2019jzzy010817)
    • Principle Award Recipient: Yu-ZhongZhang
  • the National Key Research and Development Program of China (Award 2018YFC1406700)
    • Principle Award Recipient: Yu-ZhongZhang
  • National Natural Science Foundation of China (Award 31800107)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award U1706207)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award 31630012)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award 91851205)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award 42176156)
    • Principle Award Recipient: Hui-HuiFu
© 2022 The Authors
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Halomonas profundi sp. nov., isolated from deep-sea sediment of the Mariana Trench
Int J Syst Evol Microbiol 72, 005210 (2022); https://doi.org/10.1099/ijsem.0.005210
/content/journal/ijsem/10.1099/ijsem.0.005210
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