@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.005208, author = "Yi, Bing-fang and Xia, Ting-ting and Niu, Ming-ming and Zhao, Zi-yu and Su, Qing-yang and Ming, Hong and Nie, Guo-xing", title = "Agromyces agglutinans sp. nov., isolated from saline lake sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2022", volume = "72", number = "1", pages = "", doi = "https://doi.org/10.1099/ijsem.0.005208", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005208", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Agromyces agglutinans sp. nov.", keywords = "polyphasic taxonomy", keywords = "saline lake", eid = "005208", abstract = "A novel actinobacterium, designated strain CFH 90414T, was isolated from sediment sampled at a saline lake in Yuncheng, Shanxi, PR China. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of strain CFH 90414T were Gram-reaction-positive, aerobic and non-motile. Growth occured at 4−40 °C, pH 5.0−9.0 and in the presence of up to 0–3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CFH 90414T was a member of the genus Agromyces . The 16S rRNA gene sequence similarity analysis indicated that strain CFH 90414T was most closely related to Agromyces italicus JCM 14320T (98.07 %) and Agromyces lapidis JCM 14321T (97.18 %). The whole genome of CFH 90414T was 3.64 Mb, and showed a G+C content of 71.5 mol%. The average nucleotide identity (ANI) values and digital DNA–DNA hybridization (dDDH) values between CFH 90414T and the other species of the genus Agromyces were found to be low (ANI <78.99 % and dDDH <22.9 %). The whole-cell sugars were rhamnose, mannose, ribose, glucose and galactose. The isolate contained l-2,4-diaminobutyric acid, d-alanine, d-glutamic acid and glycine in the cell-wall peptidoglycan. The predominant menaquinone was MK-12. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 90414T is considered to represent a novel species of the genus Agromyces , for which the name Agromyces agglutinans sp. nov. is proposed. The type strain is CFH 90414T (=DSM 105966T=KCTC 49062T).", }