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Abstract

A novel canavanine-degrading bacterium, strain HB002, was isolated from rhizosphere soil of a catch crop field collected from the island of Reichenau in Konstanz, Germany, and characterized by using polyphasic taxonomy. The facultative aerobe, rod-shaped, Gram-stain-negative bacterium was oxidase- and catalase-positive. The isolate was able to grow on canavanine as a sole carbon and nitrogen source. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed highest similarities to (L22-9, 99.93 %), subsp. (ATCC 49054, 99.76 %), subsp. (DBK 11, 99.63 %), (DSM 13194, 99.51 %), (DSM 13647, 99.39 %) and (ATCC29736, 99.39 %). Marker gene analysis placed the strain in the intrageneric group of , subgroup . DNA–DNA hybridization and average nucleotide identity values were both under the recommended thresholds for species delineation. The predominant fatty acids of strain HB002 were C, C cyclo ω7 and C ω7. The major respiratory quinone was Q9, followed by Q8 and minor components of Q7 and Q10. Results from the phenotypic characterization showd the strain's inability to hydrolyse gelatin and to assimilate -acetyl glucosamide and a positive enzymatic activity of acid phosphatase and naphthol-AS-BI phosphohydrolase that distinguish this strain from closely related type strains. Taken together, these results show that strain HB002 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is HB002 (=DSM 112525=LMG 32336).

Funding
This study was supported by the:
  • European Research Council (Award 681777)
    • Principle Award Recipient: JörgHartig
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2022-01-24
2022-07-06
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