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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 1

sp. nov., isolated from saline-alkaline soil No Access

Abstract

A novel bacterium, designated TRT317, was isolated from saline-alkaline soil collected from the Pamir plateau in northwest China. Cells of this strain were Gram-stain-negative, aerobic rods and red-pink-coloured. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain TRT317 showed the highest sequence similarity to the type strains of (96.3 %) and (96.2 %). Growth was observed at 4–40 °C, pH 6.0–10.0 and in the presence of up to 7 % (w/v) NaCl. The major fatty acids were iso-C and summed feature 4 (iso-C I/anteiso-C B). The polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified glycolipids and five unidentified lipids. The whole-cell sugars of strain TRT317 were mannose, rhamnose, glucose, galactose, xylose, arabinose and four unidentified sugars. The sole respiratory quinone was MK-7. The genomic DNA G+C content of strain TRT317 was 47.7 mol%. The average nucleotide identity (ANI) value of strain TRT317 with was 88.3 %, which is below the standard ANI threshold for species identification (95–96 %). Combined results of physiological, genotypic, phylogenetic and chemotaxonomic analyses demonstrated that strain TRT317 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is TRT317 (=CGMCC1.18690=KCTC 82818).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Pamir plateau , polyphasic taxonomy , Pontibacter pamirensis sp. nov. and saline-alkaline soil
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31570109)
    • Principle Award Recipient: Deng-DiAn
  • Xinjiang Uygur Autonomous Region, regional coordinated Innovation Project and Shanghai Cooperation Organization Science and Technology Partnership Program (Award 2021E01018)
    • Principle Award Recipient: Wen-JunLi
  • the Open Subject of the Key Laboratory of the Autonomous Region (Award 2017D04008)
    • Principle Award Recipient: Deng-DiAn
  • Scientific Research Program of Higher Education Institution of Xinjiang (Award XJEDU2018I016)
    • Principle Award Recipient: Deng-DiAn
© 2022 The Authors
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2022-01-25
2024-12-14
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Pontibacter pamirensis sp. nov., isolated from saline-alkaline soil
Int J Syst Evol Microbiol 72, 005200 (2022); https://doi.org/10.1099/ijsem.0.005200
/content/journal/ijsem/10.1099/ijsem.0.005200
/content/journal/ijsem/10.1099/ijsem.0.005200
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