@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.005198, author = "Chen, Xiao-Hui and Chen, Ming-Sheng and Li, Fei-Na and Tuo, Li", title = "Stakelama flava sp. nov., a novel endophytic bacterium isolated from a branch of Kandelia candel", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2022", volume = "72", number = "1", pages = "", doi = "https://doi.org/10.1099/ijsem.0.005198", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005198", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "endophytic bacterium", keywords = "Kandelia candel", keywords = "taxonomic position", keywords = "Stakelama flava", eid = "005198", abstract = "A Gram-stain-negative, aerobic, motile, non-spore-forming, short-rod-shaped strain that did not produce diffusible pigment, designated CBK3Z-3T, was isolated from a branch of Kandelia candel, collected from the Beilun Estuary National Nature Reserve in Guangxi Zhang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position. Strain CBK3Z-3T grew at pH 5.0–10.0 (optimum, pH 8.0), 20–37 °C (optimum, 25–30 °C) and with 0–10 % (w/v) NaCl (optimum, 2–3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBK3Z-3T was closely related to species of genus Stakelama and had the highest 16S rRNA gene sequence similarity of 98.7 % to Stakelama pacifica CGMCC 1.7294T. The DNA G+C content value of strain CBK3Z-3T was 62.6 mol%. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid and the polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, an unidentified aminolipid and an unidentified lipid. The major fatty acids were C18 : 1  ω7c and C16 : 0. The average nucleotide identity, estimated digital DNA–DNA hybridization and average amino acid identity values between strain CBK3Z-3T and the type strain of Stakelama pacifica CGMCC 1.7294T were 80.4, 23.1 and 81.5 %, respectively. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain CBK3Z-3T should be designated as a novel species of the genus Stakelama , for which the name Stakelama flava sp.nov. is proposed. The type strain is CBK3Z-3T (=JCM 34534T=CGMCC 1.18972T).", }