1887

Abstract

A Gram-stain-negative, aerobic, motile, non-spore-forming, short-rod-shaped strain that did not produce diffusible pigment, designated CBK3Z-3, was isolated from a branch of , collected from the Beilun Estuary National Nature Reserve in Guangxi Zhang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position. Strain CBK3Z-3 grew at pH 5.0–10.0 (optimum, pH 8.0), 20–37 °C (optimum, 25–30 °C) and with 0–10 % (w/v) NaCl (optimum, 2–3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBK3Z-3 was closely related to species of genus and had the highest 16S rRNA gene sequence similarity of 98.7 % to CGMCC 1.7294. The DNA G+C content value of strain CBK3Z-3 was 62.6 mol%. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid and the polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, an unidentified aminolipid and an unidentified lipid. The major fatty acids were C 7 and C. The average nucleotide identity, estimated digital DNA–DNA hybridization and average amino acid identity values between strain CBK3Z-3 and the type strain of CGMCC 1.7294 were 80.4, 23.1 and 81.5 %, respectively. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain CBK3Z-3 should be designated as a novel species of the genus , for which the name sp.nov. is proposed. The type strain is CBK3Z-3 (=JCM 34534=CGMCC 1.18972).

Funding
This study was supported by the:
  • Science and Technology Foundation of Guizhou Province (Award Qian Ke He Jichu [2019]1347)
    • Principle Award Recipient: LiTuo
  • National Natural Sciences Foundation of China (Award 81960642)
    • Principle Award Recipient: TuoLi
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2022-01-21
2022-05-18
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