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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 12

sp. nov., isolated from spent mushroom substrates No Access

Abstract

In Japan, during a screening of lactic acid bacteria in spent mushroom substrates, an unknown bacterium was isolated and could not be assigned to any known species. Strain YK48G is Gram-stain-positive, rod-shaped, non-motile, non-spore-forming and catalase-negative. The isolate grew in 0–4 % (w/v) NaCl, at 15–37 °C (optimum, 30 °C) and at pH 4.0–8.0 (optimum, pH 6.0). The genomic DNA G+C content of strain YK48G was 42.5 mol%. Based on its 16S rRNA gene sequence, strain YK48G represented a member of the genus and showed the highest pairwise similarity to DSM 19907 (97.86 %). Phylogenetic analyses based on amino acid sequences of 466 shared protein-encoding genes also revealed that the strain was phylogenetically positioned in the genus but did not suggest an affiliation with previously described species. The average nucleotide identity and digital DNA–DNA hybridization values between strain YK48G and the type strains of phylogenetically related species were 72.2–76.6% and 19.0–21.2 %, respectively, indicating that strain YK48G represents a novel species within the genus . Phenotypic data further confirmed the differentiation of strain YK48G from other members of the genus . According to the results of the polyphasic characterization presented in this study, strain YK48G represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YK48G (=JCM 32598=DSM 107968).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): firmicutes , lactic acid bacteria , Lentilactobacillus fungorum and spent mushroom substrates
Funding
This study was supported by the:
  • Genebank Project (Microorganism Section)
    • Principle Award Recipient: MasanoriTohno
  • NARO Gender Equality Program
    • Principle Award Recipient: HisamiKobayashi
  • NARO Gender Equality Program
    • Principle Award Recipient: MasanoriTohno
  • NARO and NIG JOINT (2016, 2017)
    • Principle Award Recipient: MasanoriTohno
© 2021 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005184
2021-12-16
2024-04-25
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Lentilactobacillus fungorum sp. nov., isolated from spent mushroom substrates
Int J Syst Evol Microbiol 71, 005184 (2021); https://doi.org/10.1099/ijsem.0.005184
/content/journal/ijsem/10.1099/ijsem.0.005184
/content/journal/ijsem/10.1099/ijsem.0.005184
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