1887

Abstract

A Gram-stain positive, strictly aerobic, non-motile and rod-shaped strain, NJ-Z5, was isolated from a soil sample obtained from the Antarctic Peninsula. This strain was taxonomically characterized by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NJ-Z5 belonged to the genus and showed the highest similarities to ALL (98.07%), followed by 4J27 (98.00%), Sphe3 (97.93%) and YJ56 (97.82%). The strain was able to grow at 4–28 °C (optimum, 25 °C), at pH 6.0–8.0 (optimum, pH 7.0) and with 0–1.0% (w/v) NaCl (optimum, 0%). It had catalase activity but no oxidase activity. The chemotaxonomic characteristics of strain NJ-Z5, which had MK-9 (H) as its predominant menaquinone and anteiso-C (58.5%), anteiso-C (9.9%) and iso-C (7.0%) as its major fatty acids, were consistent with classification in the genus . The polar lipid profile of strain NJ-Z5 comprised phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, three unidentified glycolipids and two unidentified phospholipids. The genome of strain NJ-Z5 was 4.57 Mbp with a G+C content of 67.1 mol%. Average nucleotide identity (ANI) values between strain NJ-Z5 and other species of the genus were found to be low (ANIm <86%, ANIb <80% and OrthoANIu <80 %). Furthermore, digital DNA–DNA hybridization (dDDH) and average amino acid identity (AAI) values between strain NJ-Z5 and the closely related species ranged from 22.7 to 24.0% and from 75.5 to 77.2%, respectively. On the basis of its differential physiological properties, chemotaxonomic characteristics and low ANI, dDDH and AAI results, strain NJ-Z5 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is NJ-Z5 (=CGMCC 1.15636=KCTC 39722).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award NSFC, grant number 31070002)
    • Principle Award Recipient: JianliZhang
  • National Key Research and Development Program of China (Award 2016YFC0501302)
    • Principle Award Recipient: JianliZhang
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2022-01-17
2022-07-06
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