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Volume 72, Issue 1

sp. nov., an endophytic actinomycete isolated from the root of No Access

Abstract

An endophytic -like micro-organism, designated strain PRB2-1 was isolated from root tissue of (Linden and André) G.S. Bunting. The typical morphological and chemotaxonomic characteristics, i.e. the ability to produce straight spore chains directly on aerial mycelium and the presence of -diaminopimelic acid in cell-wall peptidoglycan, were consistent with its assignment to the genus . 16S rRNA gene analysis showed that strain PRB2-1 is a member of the genus with the highest similarity to DSM 42183 (98.4 %). Moreover, the draft genome sequence of strain PRB2-1 exhibited low average nucleotide identity by (79.9–83.8 %) and digital DNA–DNA hybridization (24.9–28.3 %) values to the reference strains, which were well below the species circumscription threshold. The DNA G+C content of genomic DNA was 73.6 mol%. Comparison of phenotypic characteristics and whole-genome sequence between strain PRB2-1 and its close relatives indicated that strain PRB2-1 could be classified as a novel species of the genus . Thus the name, sp. nov. is proposed for the strain. The type strain is PRB2-1 (=TBRC 7642=NBRC 113169).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , endophytic actinomycete , Epipremnum aureum , Streptomyces and whole-gemone analysis
Funding
This study was supported by the:
  • Faculty of Science at King Mongkut's Institute of Technology Ladkrabang (Award 2562-01-05-47)
    • Principle Award Recipient: ChittiThawai
© 2022 The Authors
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2022-01-17
2024-04-19
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Streptomyces epipremni sp. nov., an endophytic actinomycete isolated from the root of Epipremnum aureum
Int J Syst Evol Microbiol 72, 005179 (2022); https://doi.org/10.1099/ijsem.0.005179
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