1887

Abstract

Two novel Gram-stain-negative bacterial strains, Azo-3 and Azo-2, were isolated from a toluene-producing enrichment culture that originated from contaminated groundwater at a site in southeast Louisiana (USA). Cells are non-spore forming straight to curved rods with single polar flagella. Strains Azo-3 and Azo-2 are oxidase-positive, catalase-negative, use nitrate and nitrite as electron acceptors, and are able to fix nitrogen. Poly-β-hydroxybutyrate storage granules are produced. Dominant fatty acids when grown in R2A medium at 37 °C are C, summed feature 3 (C ω7 and/or C iso 2OH), C cyclo and C ω7. 16S rRNA gene sequence based phylogenetic analysis indicated that the strains cluster within the family , class , most closely related to but distinct from type strains of the species (96.94% similarity) and (95.10% similarity). Complete genome sequences determined for strains Azo-3 and Azo-2 revealed DNA G+C content of 62.70 mol%. Genome-wide comparisons based on average nucleotide identity by orthology and estimated DNA–DNA hybridization values combined with phenotypic and chemotaxonomic traits and phylogenetic analysis indicate that strains Azo-3 and Azo-2 represent a novel species within the genus for which the name sp. nov. is proposed. The type strain of is Azo-3 (=NRRL B-65590=DSM 112046).

Funding
This study was supported by the:
  • Louisiana Board of Regents (Award BOR#15)
    • Principle Award Recipient: WilliamM Moe
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2021-12-16
2022-01-17
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