1887

Abstract

Four bacterial strains were isolated from two different colony sources of the wax moth . They were characterized by a polyphasic approach including 16S rRNA gene sequence analysis, core-genome analysis, average nucleotide identity (ANI) analysis, digital DNA–DNA hybridization (dDDH), determination of G+C content, screening of antibiotic resistance genes, and various phenotypic analyses. Initial analysis of 16S rRNA gene sequence identities indicated that strain GAL7 was potentially very closely related to and , having 99.5–99.9 % sequence similarity. However, further analysis of whole genome sequences revealed a genome size of 3.69 Mb, DNA G+C content of 42.35 mol%, and low dDDH and ANI values between the genomes of strain GAL7 and closest phylogenetic relative NBRC 100478 of 59.0 and 94.5 %, respectively, indicating identification of a putative new species. In addition, all novel strains encoded the atypical vancomycin-resistance gene . Results of phylogenomic, physiological and phenotypic characterization confirmed that strain GAL7 represented a novel species within the genus , for which the name sp. nov. is proposed. The type strain is GAL7 (=DSM 112306=NCTC 14608).

Funding
This study was supported by the:
  • Wellcome Trust (Award 220876/Z/20/Z)
    • Principle Award Recipient: LindsayJ Hall
  • Wellcome Trust (Award 100974/C/13/Z)
    • Principle Award Recipient: LindsayJ Hall
  • Biotechnology and Biological Sciences Research Council (Award BB/R012490/1)
    • Principle Award Recipient: LindsayJ Hall
  • Wellcome Trust (Award 110072/Z/15/Z)
    • Principle Award Recipient: AnthonyMaxwell
  • Biotechnology and Biological Sciences Research Council (Award BB/P012523/1)
    • Principle Award Recipient: AnthonyMaxwell
  • National Centre for the Replacement, Refinement and Reduction of Animals in Research (Award NC/R001782/1)
    • Principle Award Recipient: HarrietCC Gooch
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2021-12-17
2024-03-29
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