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Abstract

An actinobacterial strain designated MMS20-R1-14 was isolated from a riverside soil sample. Colonies on agar plates were orange to strong orange brown in colour, which later became black. The cells grew at 10–40 °C (optimum, 37 °C), pH 5.0–11.0 (pH 8.0) and in the presence of 0–4 % NaCl (1 %). The 16S rRNA gene sequence of strain MMS20-R1-14 showed highest similarities to CCTCC AA 2012002 (99.51 %) and AM105 (99.37 %). The orthoANI values between strain MMS20-R1-14 and the two type strains were 95.72 and 90.99 %, and the digital DNA–DNA hybridization values were 63.6 and 40.8 %, respectively, thus confirming the distinction of strain MMS20-R1-14 from its mostly related species. The DNA G+C content of strain MMS20-R1-14 was 72.9 mol%. The strain contained -diaminopimelic acid as the major cell-wall amino acid, and the characteristic whole-cell sugars were arabinose, xylose, glucose, ribose and rhamnose. The main cellular fatty acids were C 9, iso-C and iso-C, the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the predominant menaquinones were MK-10(H) and MK-10(H), all of which were consistent with those of . Strain MMS20-R1-14 showed antimicrobial activity against a range of bacterial and yeast species. The genome of the strain was found to contain 33 potential biosynthetic gene clusters for secondary metabolites, thus showing a high potential as a producer of bioactive compounds. On the basis of these phenotypic, genotypic and chemotaxonomic data, strain MMS20-R1-14 merits recognition as representing a novel species of the genus , for which the name sp. nov. (type strain=MMS20 R1-14=KCTC 49541=JCM 34494) is proposed.

Funding
This study was supported by the:
  • National Institute of Biological Resources
    • Principle Award Recipient: SeungBum Kim
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2021-12-16
2022-01-28
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