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Abstract

A novel thermophilic bacterium, designated SCSIO 07484, was isolated from marine sediment sampled in the South China Sea. Growth occurred at 30–60 °C, pH 6.0–8.0 and in the presence of 0–3 % (w/v) NaCl. Cells of strain SCSIO 07484 were rod-shaped and flagellum-forming. No soluble pigment was observed. The phylogenetic analysis of the 16S rRNA gene sequences indicated that SCSIO 07484 belonged to the family and clustered with members of the genus in the phylogenetic trees with less than 96.2 % similarities. The cell wall contained -diaminopimelic acid. Whole-cell hydrolysates contained arabinose, glucose and ribose. The predominant menaquinone was MK-7. Major fatty acids were iso-C, iso-C, C and iso-C. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine were its diagnostic polar lipids. The whole genome size of strain SCSIO 07484 was 4 079 826 bp with a DNA G+C content of 56.2 mol%, including one circular chromosome of 3 978392 bp and one plasmid of 101434 bp. Based on the polyphasic analysis of strain SCSIO 07484, it is considered to represent a novel species of the genus , for which the name sp. nov. is proposed with the type strain SCSIO 07484 (=DSM 106769=CGMCC 1.15814).

Funding
This study was supported by the:
  • academy of south china sea ecology and environmental engineering, chinese academy of sciences (Award ISEE2018ZD02)
    • Principle Award Recipient: Li-JuanLong
  • guangdong province introduction of innovative r&d team (Award 2019BT02Y262)
    • Principle Award Recipient: Xin-PengTian
  • hainan provincial department of science and technology (Award ZDKJ202018)
    • Principle Award Recipient: Xin-PengTian
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2021-12-14
2022-01-28
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