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Volume 71, Issue 12

sp. nov. and sp. nov., isolated from freshwater habitats No Access

Abstract

Two bacterial strains, designated HSP-20 and CCP-1, isolated from freshwater habitats in Taiwan, were characterized by polyphasic taxonomy. Both strains were Gram-stain-negative, aerobic, non-motile and rod-shaped. Cells of strains HSP-20 and CCP-1 formed pink and dark red coloured colonies, respectively. Both strains contained bacteriochlorophyll , and showed optimum growth under anaerobic conditions by photoheterotrophy, but no growth by photoautotrophy. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences indicated that both strains belonged to the genus . Analysis of 16S rRNA gene sequences showed that strains HSP-20 and CCP-1 shared 98.3 % sequence similarity and were closely related to CYK-10 (96.0 %) and SYSU G03088 (96.0 %), respectively. Both strains shared common chemotaxonomic characteristics including Q-10 as the major isoprenoid quinone, C 7 as the predominant fatty acid, and phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine as the main polar lipids. The DNA G+C content of both strains was 66.2 mol%. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between these two novel isolates and their closest relatives were below the cut-off values of 95–96, 90 and 70 %, respectively, used for species demarcation. On the basis of phenotypic and genotypic properties and phylogenetic inference, both strains should be classified as novel species within the genus , for which the names sp. nov. (=BCRC 81193=LMG 31334) and sp. nov. (=BCRC 81189=LMG 31335) are proposed.

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Keyword(s): alphaproteobacteria , Rhodobacter amnigenus sp. nov. , Rhodobacter ruber sp. nov. , Rhodobacteraceae , Rhodobacterales and taxonomy
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2021-12-14
2024-04-24
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Rhodobacter amnigenus sp. nov. and Rhodobacter ruber sp. nov., isolated from freshwater habitats
Int J Syst Evol Microbiol 71, 005150 (2021); https://doi.org/10.1099/ijsem.0.005150
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