1887

Abstract

A bacterial strain, named For3, was isolated from forest soil sampled in Champenoux, France. Based on its 16S rRNA gene sequence, the strain was affiliated to the family and, more specifically, to the genus . The strain had 99.93% 16S rRNA gene sequence similarity to its closest relative strains ATCC 33331, ATCC 27416, NRRL ISP-5322 and NRRL B-16504. The phylogenomic tree using the genome distance phylogeny method showed that the closest relative strain was NRRL ISP-5137 and that For3 represents a new branch among the . Genome relatedness indexes revealed that the average nucleotide identity and digital DNA–DNA hybridization values between For3 and its closest phylogenomic relative ( NRRL ISP-5137) were 88.39 and 39.2 %, respectively. The G+C content of the genome was 71.4 mol% and its size was 7.96 Mb with 7492 protein-coding genes. Strain For3 harboured complete metabolic pathways absent in the closest relative strains such as cellulose biosynthesis, glycogen degradation I, glucosylglycerate biosynthesis I. Anteiso-C, iso-C, anteiso-C and MK-9(H4)/MK-9(H6) were the predominant cellular fatty acids and respiratory quinones, respectively. Phenotypic and genomic data supported the assignment of strain For3 to a novel species sp. nov., within the genus , for which the type strain is For3 (=CIP 111908=LMG 32186).

Funding
This study was supported by the:
  • la fondation de france et la fondation du site paris-reims
    • Principle Award Recipient: NotApplicable
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-12-10
2022-01-28
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