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Volume 71, Issue 12

sp. nov., derived from human oropharyngeal abscess puncture fluid No Access

Abstract

An obligately anaerobic strain, designated as A2931, was isolated from oropharyngeal abscess puncture fluid of a patient sampled during routine care at a hospital and further characterized both phenotypically, biochemically and genotypically. This Gram-negative rod-shaped bacterium was moderately saccharolytic and proteolytic. Phylogenetic analyses of full-length 16S rRNA gene and whole-genome sequences revealed it to be best placed in the genus , but to be only comparatively distantly related to recognized species, with the closest relationship to (average nucleotide identity and digital DNA–DNA hybridization values both well below the generally accepted thresholds). Strain A2931 had a genomic DNA G+C content of 47.7 mol%. Its most abundant cellular long-chain fatty acids were anteiso-C, iso-C and C. Taken together, this polyphasic data suggests strain A2931 to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is A2931 (=DSM 108028=CCOS 1232=CCUG 72806). Interestingly, we found strain A2931 to correspond to the oral taxon HMT-820 in the Human Oral Microbiome Database, as supported by overall genome relatedness index analyses >99 %. Thus, our work not only closes one of the gaps of knowledge about hitherto unnamed species isolated from humans, but also will facilitate identification of this taxon both in the clinical microbiology context and in research alike.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteroidetes , clinical isolate , novel species , phylogenomics , proteome-based phylogenomic analysis and whole-genome sequencing
© 2021 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005146
2021-12-15
2024-04-23
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Prevotella illustrans sp. nov., derived from human oropharyngeal abscess puncture fluid
Int J Syst Evol Microbiol 71, 005146 (2021); https://doi.org/10.1099/ijsem.0.005146
/content/journal/ijsem/10.1099/ijsem.0.005146
/content/journal/ijsem/10.1099/ijsem.0.005146
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