%0 Journal Article %A Pang, Fa-Hu %A Yang, Hui-Ying %A Sun, Jie %A Yu, Xing %A Zhang, Hao %T Ottowia caeni sp. nov., a novel phenylacetic acid degrading bacterium isolated from sludge %D 2021 %J International Journal of Systematic and Evolutionary Microbiology, %V 71 %N 12 %@ 1466-5034 %C 005144 %R https://doi.org/10.1099/ijsem.0.005144 %K Ottowia caeni %K polyphasic taxonomy %K phenylacetic acid %K phylogenetic analysis %K 16S rRNA gene %I Microbiology Society, %X A novel bacterium, designated BD-1T, was isolated from a sludge sample. Cells of the novel Gram-stain-negative strain were identified to be facultative anaerobic, non-motile and short rod-shaped. Growth occurred at 15–37 °C (optimum, 30 °C), pH 5.0–10.0 (pH 7.0) and in 0–4.0  % NaCl (2.0 %, w/v). The 16S rRNA gene sequence of strain BD-1T showed the highest sequence similarity to Ottowia thiooxydans DSM 14619T (97.0 %), followed by Ottowia pentelensis DSM 21699T (96.3 %) and less than 96 % to other related strains. The phylogenetic trees revealed that strain BD-1T clustered within the genus Ottowia . Summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c, 48.2 %), C16 : 0 (23.2 %) and summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c, 8.6 %) were the major fatty acids (>5 %), and ubiquinone-8 was the respiratory quinone. Phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylglycerol were identified as the major polar lipids. Meanwhile, the G+C content of the DNA was 63.6 mol% based on the draft genome analysis. The average nucleotide identity and digital DNA–DNA hybridization values between strain BD-1T and DSM 14619T were 74.5 and 21.4  %, respectively. In addition, the novel strain completely degraded 500 mg l−1 phenylacetic acid within 72 h under the condition of 3 % NaCl. Given the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain BD-1T was considered to represent a novel species of the genus Ottowia , for which the name Ottowia caeni sp. nov. is proposed. The strain is a potential resource for the bioremediation of phenylacetic acid contaminated water. The type strain is BD-1T (=CGMCC 1.18541T=KCTC 82183T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005144