1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain YQF-2, was isolated from coastal sediment sampled in Jiangsu Province and characterized phylogenetically and phenotypically. Optimal bacterial growth occurred at 28 °C (range 4–38 °C) and pH 7 (pH 6–10). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YQF-2 was related to members of the genus and shared the highest sequence identities with KMM 1406 (98.6%), followed by H29 (98.4%), KMM 9513 (98.3%), SW-353 (98.3%), E48 (97.8%) and E49 (97.7%). The 16S rRNA gene sequence identities between strain YQF-2 and other members of the genus were below 97.2%. The digital DNA–DNA hybridization value between strain YQF-2 and KMM 1406 was 23.3±2.3%. The average nucleotide identity value between strain YQF-2 and KMM 1406 was 79.7%. The unique respiratory quinone was ubiquinone-8. Phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids. The strain had summed feature 3 (C 7 and/or C 6), summed feature 8 (C 7 and/or C 6), C, C 3-OH and iso-C 3-OH as major fatty acids. The G+C content of the genomic DNA was 50.0 mol%. On the basis of phenotypic, genotypic and phylogenetic evidence, strain YQF-2 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain YQF-2 (=KCTC 72184=MCCC 1K03663).

Funding
This study was supported by the:
  • Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (Award 1630042017019)
    • Principle Award Recipient: PuJinji
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2021-12-09
2022-01-28
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