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Volume 71, Issue 12

sp. nov., an indole acetic acid-producing endophyte isolated from a root of No Access

Abstract

A Gram-stain-positive, facultatively anaerobic, spore-forming, motile with unipolar biflagella, rod-shaped, indole acetic acid-producing bacterium, named LD4P30, was isolated from a root of collected in Inner Mongolia, northern China. Strain LD4P30 grew at pH 6.0–11.0 (optimum, pH 7.0), 10–40 °C (35 °C) and in the presence of 1–15% (w/v) NaCl (5%). The strain was positive for oxidase and negative for catalase. The major cellular fatty acids of strain LD4P30 were iso-C, C 5 and anteiso-C; the major polar lipids were diphosphatidylglycerol and phosphatidylglycerol; and menaquinone-7 was the only respiratory quinone. The genomic DNA G+C content was 36.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain LD4P30 clustered with TP2-8, YIM 91119 and BH312, and showed 99.0, 98.9, 98.0 and <97.7% 16S rRNA gene similarity to TP2-8, YIM 91119, BH312 and all other current type strains, respectively. The digital DNA–DNA hybridization and average nucleotide identity based on values between strain LD4P30 and YIM 91119, TP2-8 and BH312 were 44.9, 44.7 and 44.4%, and 91.1, 91.0 and 90.8%, respectively. Based on its phenotypic, physiological and phylogenetic characteristics, strain LD4P30 represents a novel species, for which the name is proposed. The type strain is LD4P30 (=CGMCC 1.17697=KCTC 82375).

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Keyword(s): Gracilibacillus , phylogenetic analysis , Suaeda salsa and taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31960020)
    • Principle Award Recipient: Ji-QuanSun
© 2021 The Authors
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2021-12-08
2024-04-25
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Gracilibacillus suaedae sp. nov., an indole acetic acid-producing endophyte isolated from a root of Suaeda salsa
Int J Syst Evol Microbiol 71, 005140 (2021); https://doi.org/10.1099/ijsem.0.005140
/content/journal/ijsem/10.1099/ijsem.0.005140
/content/journal/ijsem/10.1099/ijsem.0.005140
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