@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.005135, author = "Kutsuna, Ryo and Mashima, Izumi and Miyoshi-Akiyama, Tohru and Muramatsu, Yuki and Tomida, Junko and Kawamura, Yoshiaki", title = "Chryseobacterium lecithinasegens sp. nov., a siderophore-producing bacterium isolated from soil at the bottom of a pond", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2021", volume = "71", number = "12", pages = "", doi = "https://doi.org/10.1099/ijsem.0.005135", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005135", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "lecithinase", keywords = "Chryseobacterium lecithinasegens", keywords = "multilocus sequence analysis", keywords = "siderophore", eid = "005135", abstract = "Bacterial strain PAGU 2197T, which was isolated from soil collected from the bottom of a pond in Japan, is characterized in this study. Cells of strain PAGU 2197T were aerobic, Gram-negative, short rod-shaped, non-motile, flexirubin-producing, oxidase-positive, catalase-positive and lecithinase-negative. A phylogenetic study based on 16S rRNA gene sequences and multilocus sequence analysis (gyrB, rpoB and rpoD) indicated that strain PAGU 2197T belongs to the genus Chryseobacterium and is a member of an independent lineage including Chryseobacterium tructae CCUG 60111T (sequence similarity, 95.9 %), Chryseobacterium lactis CCUG 60566T (93.4 %) and Chryseobacterium viscerum CCUG 60103T (91.6 %). The average nucleotide identity values were 80.83–85.04 %. Because average nucleotide identity values of 95–96 % exceed the 70 % DNA–DNA hybridization cutoff value for species discrimination, strain PAGU 2197T represents a novel species in the genus Chryseobacterium . The genome of strain PAGU 2197T was 4 967 738 bp with a G+C content of 35.5 mol%. The sole respiratory quinone of strain PAGU 2197T was MK-6; the major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3OH, summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c) and summed feature 9 (iso-C17 : 1  ω9c and/or C16 : 0 10-methyl); and the major polar lipids were phosphoglycolipids and phosphatidylethanolamine. These results indicate that strain PAGU 2197T should be classified as representing a novel species in the genus Chryseobacterium , for which the name Chryseobacterium lecithinasegens sp. nov. is proposed, with strain PAGU 2197T (=NBRC 114264T=CCUG 75150T) as the type strain.", }