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Volume 71, Issue 12

gen. nov., sp. nov., isolated from rhizospheric soil of a pine tree No Access

Abstract

A Gram-stain-negative, aerobic and rod-shaped novel bacterial strain, designated MAH-26, was isolated from rhizospheric soil of a pine tree. The colonies were orange coloured, smooth, spherical and 0.7–1.8 mm in diameter when grown on Reasoner's 2A (R2A) agar for 2 days. Strain MAH-26 was able to grow at 10–40 °C, at pH 6.0–9.0 and with 0–1.0 % NaCl. Cell growth occurred on nutrient agar, R2A agar, tryptone soya agar and Luria–Bertani agar. The strain gave positive results in oxidase and catalase tests. Strain MAH-26 was closely related to CJ663 and SGM2-10 with a low 16S rRNA gene sequence similarity (92.8 and 92.9 %, respectively) and phylogenetic analysis indicated that the strain formed a distinct phylogenetic lineage from the members of the closely related genera of the family . Strain MAH-26 has a draft genome size of 6 857 405 bp, annotated with 5173 protein-coding genes, 50 tRNA and two rRNA genes. The genomic DNA G+C content was 41.5 mol%. The predominant isoprenoid quinone was menaquinone 7. The major fatty acids were identified as iso-C, iso-C G and iso-C 3OH. On the basis of phylogenetic inference and phenotypic, chemotaxonomic and molecular properties, strain MAH-26 represents a novel species of a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is MAH-26 (=KACC 19749=CGMCC 1.13701).

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Keyword(s): 16s rRNA , fatty acid , genome sequence and Pinibacter aurantiacus
© 2021 The Authors
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2021-12-06
2024-04-19
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Pinibacter aurantiacus gen. nov., sp. nov., isolated from rhizospheric soil of a pine tree
Int J Syst Evol Microbiol 71, 005132 (2021); https://doi.org/10.1099/ijsem.0.005132
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