sp. nov., a novel butyrate-producing bacteria isolated from faeces of a patient with Crohn's disease No Access

Abstract

Cultivation and isolation of gut bacteria are necessary for understanding their role in the intestinal ecosystem. We isolated a novel bacterium, designated strain BG01, from the faeces of a patient with Crohn's disease. Strain BG01 was a strictly anaerobic, rod-shaped, Gram-variable and endospore-forming bacterium. Strain BG01 possessed C, C dimethyl aldehyde (DMA) and C 9 DMA as predominant cellular fatty acids and -diaminopimelic acid as a diagnostic diamino acid. Strain BG01 grew at 15–45 °C (optimum, 37 °C), with 0–4 % (w/v) NaCl (optimum, 0–1 %), at pH 6–10 (optimum, pH 7) and was resistant to bile salt, but not to ampicillin, metronidazole, vancomycin and cefoperazone. Butyrate, propionate, oxalacetate and fumarate were produced as fermentation end products from Gifu anaerobic medium broth. Strain BG01 showed 97.7 % 16S rRNA gene sequence similarity, and 92.0 and 48.5 % of average nucleotide identity and digital DNA–DNA hybridization values, respectively, with KCTC 15019. Genomic analysis indicated that strain BG01 had a butyrate-producing pathway. The genomic G+C content of the strain was 43.5 mol%. Results of the phenotypic, phylogenetic and genotypic analyses indicated that strain BG01 represents a novel butyrate-producing species of the genus , for which the name sp. nov. is proposed. The type strain is BG01 (=KCTC 15617=JCM 32275).

Funding
This study was supported by the:
  • Korea Research Institute of Bioscience and Biotechnology (Award KGM5232113)
    • Principle Award Recipient: Na-RiShin
  • Ministry of Science & ICT (KR) (Award NRF-2017M3A9F3046549)
    • Principle Award Recipient: Jin-WooBae
  • National Research Foundation of Korea (Award NRF-2020R1A2C3012797)
    • Principle Award Recipient: Jin-WooBae
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/content/journal/ijsem/10.1099/ijsem.0.005129
2021-12-06
2024-03-28
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