@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.005129, author = "Lee, Jae-Yun and Kang, Woorim and Shin, Na-Ri and Hyun, Dong-Wook and Kim, Pil Soo and Kim, Hyun Sik and Lee, June-Young and Tak, Euon Jung and Sung, Hojun and Bae, Jin-Woo", title = "Anaerostipes hominis sp. nov., a novel butyrate-producing bacteria isolated from faeces of a patient with Crohn's disease", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2021", volume = "71", number = "12", pages = "", doi = "https://doi.org/10.1099/ijsem.0.005129", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005129", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Crohn’s disease", keywords = "gut microbiota", keywords = "Anaerostipes hominis sp. nov.", keywords = "butyrate-producing bacteria", eid = "005129", abstract = "Cultivation and isolation of gut bacteria are necessary for understanding their role in the intestinal ecosystem. We isolated a novel bacterium, designated strain BG01T, from the faeces of a patient with Crohn's disease. Strain BG01T was a strictly anaerobic, rod-shaped, Gram-variable and endospore-forming bacterium. Strain BG01T possessed C12 : 0, C18 : 0 dimethyl aldehyde (DMA) and C18 : 1  ω9c DMA as predominant cellular fatty acids and meso-diaminopimelic acid as a diagnostic diamino acid. Strain BG01T grew at 15–45 °C (optimum, 37 °C), with 0–4 % (w/v) NaCl (optimum, 0–1 %), at pH 6–10 (optimum, pH 7) and was resistant to bile salt, but not to ampicillin, metronidazole, vancomycin and cefoperazone. Butyrate, propionate, oxalacetate and fumarate were produced as fermentation end products from Gifu anaerobic medium broth. Strain BG01T showed 97.7 % 16S rRNA gene sequence similarity, and 92.0 and 48.5 % of average nucleotide identity and digital DNA–DNA hybridization values, respectively, with Anaerostipes caccae KCTC 15019T. Genomic analysis indicated that strain BG01T had a butyrate-producing pathway. The genomic G+C content of the strain was 43.5 mol%. Results of the phenotypic, phylogenetic and genotypic analyses indicated that strain BG01T represents a novel butyrate-producing species of the genus Anaerostipes , for which the name Anaerostipes hominis sp. nov. is proposed. The type strain is BG01T (=KCTC 15617T=JCM 32275T).", }